PCR体系构建

A Freedom EVO® platform is helping scientists at Italy’s Stazione Zoologica Anton Dohrn, one of the world’s top marine biology and ecology research institutions, to provide a range of automated genomics services for marine biologists.

The Dittmer Lab at the University of North Carolina is investigating the biology of viral cancers using a Freedom EVO® 150 with an integrated LightCycler® 480 Real-Time PCR System. This versatile set-up provides automated qPCR-based analysis for viral gene and microRNA expression profiling, as well as viral load testing of clinical trial samples.

Repetitive manual pipetting and tedious serial dilutions for drug discovery assays just became a thing of the past. With Tecan’s D300e Digital Dispenser, you can automatically dispense any volume into any well of any assay plate, quickly and easily. Simply add your liquid to the Dispensehead and let the instrument do the rest.

The TouchTools™ PCR Wizard offers straightforward automation of PCR reaction set-up on the Freedom EVO® PCR workstation, using the high precision Air displacement pipetting arm (Air LiHa).

Fully automated reaction set-up for complex PCR plate layouts, with up to 96 master mixes and multiple PCR plates per run, plus individual controls and dilution curves.

Fluent’s groundbreaking design delivers more capacity and increased speed for genomic workflows. This innovative solution offers superior precision, higher throughput and longer walkaway times. Get more done in less time, with greater confidence.

Automate more of your genomics workflows – including reaction set-up for endpoint PCR or qPCR, hybridization, Sanger or next generation sequencing – with a dedicated on-deck cycler from INHECO.

This Application Note describes automated qPCR set-up with the KAPA™SYBR® FAST qPCR Kit on the Fluent® Automation Workstation with minimal hands-on time. In the TouchTools™ user interface, the number of samples, master mixes or replicates can be chosen from a drop-down menu or input manually. Optional procedures include generation of a standard curve with a variable number of dilution points. Typical set-up times are 25 min for a 96-well plate.

DNA profiling of endangered species by PCR at the Institute for Environmental Protection and Research.

The application note shows that the D300e is a suitable tool for qPCR set-up, offers significant sample, primer and reagent savings, and greatly reduces workflow time.

The data presented in this application note demonstrates that the D300e can be used to miniaturize qPCR reactions to volumes as low as 2 μl and in addition offers greater process security and speed of the automated set-up.