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细胞生物学

细胞系和原代细胞广泛用于生产细胞衍生的生物分子,以及用于基于细胞的测定。

Tecan提供复杂的模块化的自动解决方案,从2D和3D带克隆的细胞培养、转染和集落挑选到基于细胞的测定和基于我们的液体处理和检测平台的细胞分析。

概观

基于细胞的筛选在药物发现过程中起重要作用。 自动化有助于加速铅化合物的选择和细胞靶点的分析。 运行强大的基于细胞的测定能力是筛选活动成功的关键。Tecan提供模块化解决方案,适用于各种基于细胞的测定,从极低到极高的通量筛选,只需一种仪器即可进行测定开发和常规筛选。

主要优点

  • 使用自动化加速您的基于细胞的测定
  • 引入标准化,实现最佳效果
  • 整合上下游应用

细胞健康

简单的剂量反应实验设置,包括结果分析。

Tecan提供了模块的理想组合以优化试剂和细胞制备,可以独立于所用分析技术(荧光,比色或光度),对细胞活性,细胞毒性或死细胞进行强大和可重复的评估。使用任意Tecan液体处理平台可轻易设置用于剂量反应实验的连续稀释,而使用 D300e 超微量加样仪可以轻松制备更复杂的复板。

为了工作流程的完全自动化,可以将细胞板存储在自动环境控制CO2培养箱中,并可以添加诸如振动器,垫圈,实验室器皿转盘和可弃置存储, 成像多功能酶标仪或高通量酶标仪。

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细胞信号传导/路径

调查细胞的通信 – 因自动化而更强大。

Tecan提供了基于激酶活性正交测定的强大自动化的工具,以及聚焦于细胞信号传导和途径的基于细胞筛选的G蛋白相关测定。 完全集成的层状罩也可用于为表观遗传学测定提供最佳的细胞保护,其通常具有较长的随访时间。

使用Tecan液体处理平台易于设置用于剂量反应实验的连续稀释,而使用D300e数字分配器可以轻松制备更复杂的复合板。为了工作流程的完全自动化,可以将细胞板存储在自动环境控制CO2培养箱中,并可以添加诸如振动器,垫圈,实验室器皿转盘和可弃置存储, 成像多功能酶标仪或高通量酶标仪。

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基因报告

找出哪些基因在特定的细胞事件中表达。

基因报告测定是学术界和制药公司生命科学研究的重要组成部分。 报告蛋白的表达可以通过直接检测蛋白质或通过测量其酶活性来实现。

由于这些测定通常具有较长的运行时间,因此适当的细胞保护和孵育至关重要。 为了工作流程的完全自动化,可以将细胞板存储在自动环境控制CO2培养箱中,并可以添加诸如振动器、垫圈、实验室器皿转盘和可弃置存储, 成像多功能酶标仪或高通量酶标仪。
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高通量筛选

基于细胞的高通量筛选通常用复杂的光学测定法来补充以研究细胞对小分子,miRNA,抗体或其他化合物等刺激物的反应。

Tecan提供了一系列液体处理选项,包括MCA 96MCA 384,以简化96孔,384孔和1536孔板中的测定。 创新的MCA 384还可以自动更换头部适配器以处理不同的尖端类型,数量甚至行列提示。为了工作流程的完全自动化,可以将细胞板存储在自动环境控制CO2培养箱中,并可以添加诸如振动器,垫圈,实验室器皿转盘和可弃置存储, 成像多功能酶标仪或高通量酶标仪。

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Tecan 期刊文献

The Monash Antibody Technologies Facility (MATF) in Victoria, Australia, has added a new dimension to its high throughput service, offering screening for antibody functionality. This time-consuming phase is crucial for many projects, and can now be outsourced to MATF, where comprehensive and flexible automation completes testing in a fraction of the time.

The new Spark 20M multimode microplate reader offers tailor-made solutions to suit virtually any drug discovery or advanced life science research application. This freely configurable system gives researchers access to new techniques and features intended to enhance and streamline biochemical and cell-based workflows.

The Karlsruhe Institute of Technology has automated protocols for high throughput separation of cells for therapeutic applications on a Freedom EVO® 200 liquid handling platform. The process maintains cell viability and is more rapid and reproducible than manual methods, enabling processing of 96 samples in four hours.

Scientists in the ADME Screening, Enzymology and Automation Group at Roche, Switzerland, have successfully integrated a mosquito® nanoliter liquid handling instrument with a Freedom EVO® platform, enabling accurate, precise pipetting at very low volumes.

Scientists working in the Screening Unit at the Institute for Molecular Pharmacology in Germany are finding the low volume pipetting capability of the MultiChannel Arm™ 384 beneficial for the investigation of siRNAs and small molecule compound screening.

Tecan and pION Inc. have combined pION’s PAMPA Explorer Test System™ with Tecan’s microplate readers for drug permeability screening using the Double-Sink™ parallel artificial membrane permeability assay (PAMPA™).

The German army has automated the determination of AChE activity in whole blood. The determination of cholinesterase status is primarily intended for analyzing patients poisoned with organophosphates (accidental or suicidal poisoning), but it can also be applied after the use of nerve agents in military conflicts or terrorist attacks, as well as in the context of occupational medical examinations.

视频

Fluent™ 实验室基于细胞测定的自动化解决方案。

完全的移液灵活性简化了复杂的定制协议。

迅速交换转接板,即使尖端保持连接。

通用多通道臂尖阵列可实现双重连续稀释以提高生产率。

1536孔板兼容性提供更高的通量。

黑暗中的温控孵化,而震动使复杂的基于细胞的测定自动化

概述

Tecan的多功能酶标仪包括细胞成像功能,适用于广泛的细胞检测分析。 高通量酶标仪的集成使得可以直接观察细胞对刺激的反应,而流式细胞术允许分离特定的细胞类型。

Key benefits

  • Tecan多功能和单功能酶标仪的无缝集成
  • 确保您细胞适当的环境,以达到最佳效果
  • 可扩展的解决方案 - 半自动化到高吞吐量
  • 与选定合作伙伴共同开发应用程序,以提供完整的解决方案

Spark®

从吸光度,荧光和发光测量到精确的细胞计数和基于亮场的细胞成像,发现全系列的酶标仪。

Tecan的酶标仪为您基于细胞的应用提供了广泛的数据分析方法。 Spark系列的高灵活性将所有主要的检测技术结合在一个易于使用的仪器中。

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流式细胞仪

将MACSQuant®系列仪器集成到Tecan的液体处理平台中,从您基于细胞的测定中收集有价值的数据。

细胞生物学工作流程通常需要使用定义的细胞密度以便正确解释数据。

The MACSQuant flow cytometers from Miltenyi Biotec provide comprehensive multi-parameter flow cytometric analysis using up to three lasers and 10 optical channels. All sample preparation steps – from cell dilution to reagent addition with mixing and shaking – are fully automated, before the microplates are then transferred to the MACSQuant for final analysis.

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高通量酶标仪

现在,基于细胞的高通量筛选通常用复杂的光学测定法来补充以研究细胞对小分子,miRNA,抗体或其他化合物等刺激物的反应。

自动化的高通量筛选过程,结合标准化的细胞培养过程,包括孵化,收获,电镀和培养基交换 - 有助于减少样品和用户之间的差异性。

The integration of your choice of high content reader (eg. ArrayScan™ from Thermo Scientific, IN Cell Analyzer from GE Healthcare Life Sciences, Cellavista from SynenTec) makes Tecan’s liquid handling platform a complete solution.

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Tecan期刊文献

自从2月份在SLAS 2015推出以来,Spark®10M已经在整个生命科学界获得了兴趣,全球研究人员开始探索这种令人兴奋的新系统的先进功能,包括DNA定量,微生物学研究和基于细胞测定。

新的Spark 10M多功能酶标仪旨在为细胞生物学和基因组学客户提供更大的灵活性和更高的生产力。 从微生物学研究和基于细胞的测定到快速的DNA定量,全新的平台提供高级功能的组合并且非常易于使用,以简化您的日常工作。

视频

下甲板酶标仪集成

Tecan Spark 20M提供了一种新的自动化亮场细胞成像和汇合测量功能,可提高无人值守操作

概述

从传统的单层培养到复杂的3D细胞培养和高度细腻干细胞的技术,Tecan为您提供适合您应用的工具。 培养基交换,扩展和抗体生产等常规过程都是全自动化的。

Key benefits

  • 在自然环境中生长细胞 - 自动化3D细胞培养
  • 完整单克隆抗体生产工作流程
  • 通过自动重复细胞维护任务腾出工作人员的时间
  • 通过标准化提高一致性

3D细胞培养

通过使用以3D生长的细胞,改善您基于细胞的分析,细胞形态,增殖,代谢和表达谱的生物相关性。从各种格式中选择,无论是有支架还是无支架,都可以模仿真实的体内环境。

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Scaffold-based: 3D Bloom® microtissues

CellSpring’s 3D Bloom Biopolymer Platform is a proprietary technology for producing 3D microtissues using virtually any cell type. The naturally-derived 3D Bloom biopolymers enable cell-friendly cross-linking, organizing cells into 3D structures that allow both cell-cell and cell-matrix interactions, closely mimicking the behavior of tissue in vivo. Tecan has collaborated with CellSpring to automate 3D Bloom cell culture generation on the Freedom EVO platform, combining speed and versatility with consistency and reproducibility for high throughput applications.

Scaffold-based: RAFT™ collagen scaffold

Tecan and TAP Sartorius have developed an automated process for the preparation of RAFT 3D cell cultures. RAFT is now being distributed by Lonza BioScience. The patented RAFT system offers a simple, consistent and reproducible 96-well format, providing a biologically relevant collagen matrix environment for cells. Protocols have been optimized to offer fast and reliable walkaway processing for a wide range of cell biology applications, including cell-based screening, imaging and biochemical techniques.

Scaffold-based: Alvetex® scaffold

Tecan is cooperating with ReproCELL to automate the use of the Alvetex scaffold for routine 3D cell biology on the Freedom EVO®. The Alvetex scaffold in a 96-well format can be used in any laboratory currently using 2D cell biology techniques. It provides an excellent in vivo proxy in a variety of cellular and molecular techniques and biochemical assays, as cells demonstrate enhanced cellular function and responsiveness.

Scaffold-free: GravityPLUS™

Tecan is working with InSphero, a leading supplier of organotypic 3D microtissuespheroids for biomimetic drug testing, to support scaffold-free 3D cell culture systems. Cells are grown in hanging drops of culture media using 96­well GravityPLUS™ plates, enabling the efficient and reliable formation of spheroids completely free from contact with artificial support matrices or surfaces. The 96-well GravityTRAP™ assay plate ensures the microtissue is centered within the well, offering proper optical characteristics and excellent compatibility with multimode readers, such as the Infinite® 200 PRO.

 

抗体生产

提高劳动密集型抗体生产过程的生产力。

产生针对大量抗原的抗体是一项耗时的过程,并且需要许多复杂的步骤,例如细胞融合,杂交瘤克隆选择和细胞扩增。自动化所有或甚至只是最劳动密集型的加工步骤可以显著提高生产力,使得单一设备可以每年生产数千种单克隆抗体。

细胞维护和扩张

通过专门的工具和设备自动化主要瓶颈,如培养基交换,细胞电镀,孵化和测定准备。

Culture vessels

1-1'536 well plates, tubes, RoboFlasks®, transwell plates

Robotics

Plate, lid and insert handling, tube handling, plate inclination, RoboFlask operation

Storage

High capacity CO2 incubators, workdeck incubators, consumable storage (carousels, stackers)

Imaging

Confluence measurement, cell counting

Biocontainment

Sterile environment with HEPA filter and UV protection or Class II Biosafety Cabinet.

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干细胞

自动处理精细的干细胞以获得可重复的结果。

通过将仪器置于无菌环境中,能够完全自动化具有或不具有饲养细胞的多能细胞和分化细胞的生长。综合检测装置跟踪和反应于不期望的自发分化。该系统的灵活性还使您能够吸取微量生长因子,细胞因子和其他添加剂,以及提供大量介质和缓冲液分配。

Tecan期刊文献

一场3D革命
[TJ 02/2016]

一种新颖的3D细胞培养平台使科学家能够产生与几乎任何细胞类型的人类组织行为密切相似的微量组织。这种专有技术易于自动化,为高通量应用提供高速度,多功能性,一致性和可重复性。

看得更清楚
[TJ 02/2016]

退行性眼病对世界各地的数百万人造成影响,导致视力逐渐丧失,在某些情况下,甚至完全失明。澳大利亚的研究人员正在使用干细胞技术来研究如何阻止或甚至扭转常见眼睛疾病的影响。

Karlsruhe理工学院拥有在FreedomEVO®200液体处理平台上用于治疗应用的高通量分离细胞的自动化方案。该过程保持细胞活力,并且比手动方法更快速和可重复,使得能够在四小时内处理96个样品。

Tecan和TAP Biosystems通过在FreedomEVO®工作站上自动化TAP的RAFT™细胞培养技术,创建了一个自动化的3D细胞培养筛选平台。该系统使用类似于体内的胶原基质浓度来确保在与天然组织非常相似的环境中的细胞生长和增殖,并增加了自动化液体处理的可靠性和产量。

成功的框架
[TJ 02/2013]

基于Freiburg的Oncotest有限公司专门从事临床前药理优选法研究,并在FreedomEVO®工作站上成功自动化3D细胞培养,使用了Reinnervate的聚苯乙烯基的Alvetex®Scaffold 96孔板

Tecan和InSphero开发了一种基于Infinite®M200 PRO和InSphero 3D InSight™微量组织技术以分析3D细胞培养的新颖解决方案。该解决方案旨在促进在药物筛选应用中使用3D细胞培养形式,有助于降低早期药物开发的成本。

视频

3D Bloom微组织 - 3D细胞培养中的新水平

观察水凝胶RAFT从细胞接种制备到细胞培养物的产生。

使用Alvetex脚手架进行3D细胞培养

细胞骨架结构的共聚焦成像

Tecan Spark 20M提供了一种新的自动化亮场细胞成像和汇合测量功能,可提高无人值守操作。

温和洗涤弱粘附细胞

具有条形码读取能力的高容量转盘,用于样品跟踪

将试剂置于密封容器中保持清洁。 用固定的可清洗尖端穿过隔垫可防止污染

黑暗中的温控孵化,而震动使复杂的基于细胞的测定自动化

将细胞保持悬浮在振荡器的深孔板中,而不会暂停进行移液

概述

成功的细胞生物学工作流程依赖于生成高质量的克隆。 Tecan提供从脂质转染等标准方法到专门的核转染方法的全自动转染。 随后通过集落采集或细胞分离装置的集成来选择理想的克隆。

Key benefits

  • 目标蛋白质高效转染和表达
  • 在最短的时间内选择正确的克隆
  • 减少细胞系生长发育时间
  • 与选定合作伙伴共同开发应用程序,以提供完整的解决方案

有效的集落采摘

细菌,真菌,藻类,杂交瘤和哺乳动物细胞集落的轻松可靠的采摘。

自动化您的克隆和细胞系开发工作流程中最劳动密集型和容易出错的步骤。专为FreedomEVO®平台而设计,可以将SciRobotics的Pickolo™集落采集器与NAP,PCR设置或蛋白质纯化等上下游实验无缝集成。

Key advantages are

  • fast performance – pick up to 800 colonies per hour on Freedom EVO and up to 1000 colonies per hour on Fluent
  • flexible colony selection criteria by color, size, diameter and more
  • intuitive picking profile creation – just click on the colonies you like best
  • flexible source plates – Petri dishes, SBS-formats.
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Automated spiral plating and streaking

Complete your cloning and microbiology screening workflow by integrating a fully automated, high throughput solution for Petri dish inoculation.

Manual plating can be tedious, especially if the preparation of serial dilutions is taken into account. Combining the SciRobotics PetriPlater™ with Tecan’s liquid handling platforms provides a tailored solution to accelerate plating and free up staff time.

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Spiral plating

Set up various dilutions in a single plate for colony counting:

  • Uniform rotation speed – to achieve a 1:6 dilution
  • Accelerating rotation speed – to provide dilution ratios of 1:20 to 1:50
  • Decelerating rotation speed – to achieve uniform sample distribution
  • Segmented plating – to create different sample dilutions across a plate to save space and time

Spiral streaking

  • Spatially separated colonies for reliable colony-picking experiments
  • Work with samples in a variety of labware formats – including tubes, deep-well plates or even Petri dishes (in combination with the Pickolo™ Colony-Picker)
  • Ideal for streaking out urine, blood, swabs and other sample types

Scalable solution

  • Dedicated, compact and cost-effective solution including a Freedom EVO® 75 with robotic and single channel pipetting arms, PetriPlater and Petri dish storage
  • Add functionality – combine with the Pickolo Colony-Picker to complete your microbiology or synthetic biology workflow
  • Take advantage of a larger Freedom EVO or Fluent® workstation to integrate colony plating and streaking with other up- and downstream experiments

Save money, space and time

  • Robotic arm handles up to 60 Petri dishes per hour
  • Plate different dilutions on one Petri dish to save handling time, consumables costs and space
  • Fully automated serial dilutions for true walkaway processing

可重复转染和表达

自动化所有费时费力的转染不同细胞类型的过程,从制备细胞和转染混合物到转染板孵化。

Tecan’s workstations have a proven track record for automated transfection, no matter which method is used: lipofectamine, calcium phosphate or polyethylenimine (PEI) based transfection or Lonza’s Nucleofector® 96-well Shuttle™ system. Tecan instruments are also used for siRNA screenings.

Generation and expression of nucleic acid constructs are often based on standardized kits. Tecan works together with the most renowned kit manufacturers to jointly supply worry-free automation protocols. Whether your individual workflow solution requires a clean bench or a Class II Biosafety Cabinet, a CO2 incubator, cooling or heating devices to keep samples and reagents stable, or a multimode imaging reader, Tecan can provide you with tailored solutions.

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可靠的克隆选择

从随机生成的细胞系池中更快地找到最佳克隆。

细胞系发育是任何生物制剂治疗的核心,但是在某种程度上产生稳定的生产细胞系用于研究和生产仍然是随机的。该过程中最关键和不一致的步骤是从随机生成的细胞系池中选择最佳克隆。自动化可以应用于有限稀释电镀,板复制,评估单细胞克隆起源,克隆采摘,使用ELISA的进行表达筛选,蛋白质定量以及一批蛋白质表征方法,以减少开发时间并满足数据和过程文件的法规要求。

Automation can be applied to limited-dilution plating, plate replication, assessing single cell clone origin, clone picking, expression screening using ELISAs, protein quantification and a range of protein characterization methods in order to reduce development time and fulfill regulatory requirements for data and process documentation.

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安全的细胞分离

发现用于高通量细胞和生物分子分离和分析的独特而全面的解决方案。

Tecan和Miltenyi Biotec已经联手。可以选择在MultiMACS™Cell24 Separator Plus上分离未改变的活细胞, 或MultiMACS M96thermo分离器上以分离病毒和生物分子,如核酸或蛋白质,而不用担心交叉污染。

Key advantages are

  • seamless software integration into Freedom EVOware®
  • contactless pipetting to prevent cross contamination
  • flexible format – 24- and 96-well
  • separation of viruses, cells, nucleic acids, proteins and more.
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Tecan期刊文献

Designer proteins
[TJ 03/2015]

蒙特利尔大学的研究人员受益于Tecan与SciRobotics的合作,以自动化合成生物学的DNA装配协议。系统生物学和合成生物学研究部门结合Freedom EVO®200与Pickolo™集落采集器和各种其他组件,构建了简化的克隆工作流程,增加了生产量和可靠性。

西班牙能源制造商Repsol依靠两个FreedomEVO®平台和Tecan的350μl嵌套液体处理一次性加样针进行替代能源研究,加长时间的无人值守对此至关重要。

俄罗斯近年来一直是生物技术和生命科学快速增长的地区,粮食和农业是重点关注领域。这是来自实验室的一个快照,它们研究微生物在食品生产中的对比作用,在工作流程中都采用了Tecan设备。

实验室自动化和LIMS专家Mejoran 实验室自动化公司开发了一种用FreedomEVO®150平台进行自动真菌菌落采集的定制方法,该系统具有集成的SciRobotics Pickolo™集落采集器。这种创新的菌落采集方法为下游测定生产一致质量的活性微菌剂,挑选突变体进一步调查所需的时间从一周减少到一天。

石原三洋开发有限公司中央研究院生命科学实验室利用Tecan的FreedomEVO®200工作站开发了一种成功的自动化方案,用于有效的siRNA转染,为小RNA文库的有效高通量筛选铺平了道路。

使用FreedomEVO®系统和SciRobotics的Pickolo™集落采集器的自动化彻底改变了Evogene实验室的基因克隆和集落采集工作流程,从而实现了克隆过程的高效可靠跟踪,并使科学家能够提高克隆的年产量。

Tecan和amaxa 股份有限公司(现在的Lonza)已经将amaxaNucleofector®96孔Shuttle®系统与Tecan FreedomEVO®液体处理工作站成功整合,允许全自动,高通量转染原代细胞和难以转染的细胞系,包括非分裂神经元和T细胞,以及siRNA,DNA和其他底物。

视频

从Freedom EVO®Colco上以Piccolo™集落采摘

从96孔到384孔微孔板的精确采集

Webinar: Automate colony picking and forget about toothpicks

Brochure

Discover tailor-made solutions for your cell biology workflow from cell maintenance and cell-based assays to life cell imaging.

Cell-based assays - Brochures

Tecan has teamed up with pION Inc. to provide an automated solution for running high throughput drug permeability screening, using the pION Double-Sink™ parallel artificial membrane permeability assays (PAMPA™) and μSOL™ solubility assay. The flexibility of Tecan’s Freedom EVO® workstation and Infinite® M200 PRO microplate reader, combined with Pion’s Gut-Box™ microplate stirrer and PAMPA Evolution™ technology, provides a complete solution for drug candidate screening.

Tecan has created a range of automated ADME screening systems which maximize the efficiency and throughput of these processes. These systems are based on our liquid handling platform for automated cell permeability, compound characterization, in vitro drug metabolism and cell culturing procedures.

Discover a platform that speaks the language of your laboratory, and make your cell-based assays simpler, more efficient… and fluent.

Cell-based assays - Application notes

The data presented in this application note underlines the suitability of the D300e Digital Dispenser for protein-based assays.

The results generated with the D300e Digital Dispenser are consistent with an expertly performed manual process, but with the benefit of significant time savings and the elimination of serial dilution.

Fully automated HDAC assays on the Fluent® Laboratory Automation Solution where triplicates of 10 serially diluted compounds per plate were tested for dose response to selective histone deacetylase inhibition.

The ability to carry out cell-based G-Protein coupled receptor (GPCR) assays in high throughput is a key part of the drug discovery process. Combining a Fluent® Laboratory Automation Solution configured for cell-based assays with the DiscoverX PathHunter® β-Arrestin human and ortholog GPCR assay kits gives users the power to run 384-well plate-based assays in high throughput.

Growth inhibition (GI50) of a set of eight compounds was tested on four different NCI-60 cell lines on a Fluent® Laboratory Automation Solution.

Easy automation of Cisbio’s EPIgeneous™ cell-based assays on a Fluent® Laboratory Automation Solution. In order to discover the optimal assay conditions, four different cell densities of three different cell lines were deployed to determine the level of H3K27Me3 and total H3 under different conditions.

Increasing sample numbers drive many laboratories to look for opportunities to automate and optimize their liquid handling processes. One of the dilemmas that laboratories face when configuring their automated liquid handling systems is the need to balance capacity and functionality against space and budget constraints.

The PAMPA Explorer Test System™ implemented on Tecan´s Infinite® M200 PRO multimode microplate reader comprises a quick, reliable system that accurately models both human jejunal permeability (GIT) and rodent blood-brain barrier (BBB) uptake of candidate drug molecules, based on measurements of permeability across artificial phospholipid membranes.

Cell-based assays - Posters

Cytochrome P450 screening assay automated in a 384-well microplate format for lead generation using three different CYP enzymes.

Easy automation of a biochemical assay on the Fluent® Laboratory Automation Solution to identify coagulation factor IIa inhibitors in a 384-well plate format.

Cellular imaging and analysis - Brochure

The Spark multimode microplate reader platform off ers solutions to suit virtually any life science research or drug discovery application. It allows researchers to freely confi gure the reader to their current needs, and is fully upgradable to access other techniques and features in the future.

Cellular imaging and analysis - Application notes

This Technical Note describes the functions and benefits of the Spark multimode microplate reader and cell imaging module, as well as the SparkControl software and its dedicated Cell Counting and Cell Viability Application.

In this technical note, we demonstrate the superior performance of the Spark reader compared to two other multimode readers from different providers. The detection limit for the far red fluorescent dye Alexa Fluor 647 – which has a fluorescence spectrum comparable to Cy5 – was determined, operating each instrument in MCR mode.

Cell counting, viability analysis and determination of size distribution are routinely performed by researchers across multiple disciplines, from standard cell passaging for downstream experiments to using insect cells infected with baculovirus to produce recombinant proteins, or determining the differentiation capacity of stem cells. Tecan’s Spark multimode reader has an integrated, bright field cell imaging module. This patent-pending system enables label-free cell counting, size distribution determination and trypan blue-based cell viability analysis with an easy-to-use, disposable Cell Chip™. In this study, the analysis of cell concentration, cell viability and size distribution for the determination of the optimal protein production rate in recombinant Sf21 cell cultures is presented.

For many cell biology applications – such as the identification and separation of high expression clones or knock-down candidates, and small-scale antibody or protein production – it is important to ensure that cell lines originates from a single cell, i.e. they are monoclonal. This can be a major bottleneck, especially for academic laboratories, as dedicated automated microscopy systems are both very expensive and complex to operate, meaning verification of monoclonality is often performed by manual microscopy.

Tecan’s Spark multimode microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cell confluence, thereby boosting automation of cell-based studies.

Cell migration is a central process in the development and maintenance of multicellular organisms, with tissue formation during embryonic development, wound healing and immune responses all requiring the orchestrated movement and assembly of cells.

Cellular imaging and analysis - Posters

Until now, it has only been possible to heat multimode readers using temperature control, not to set them at ambient temperature or lower. This can cause problems for temperature-sensitive experiments, such as enzymatic assays, affecting the reproducibility of results over time. In addition, the experimental precision can be decreased by the plate heating up during measurements, even over just a few minutes. Tecan’s Te-Cool™ module for the Spark® platform, the first air conditioning system for a multimode reader, addresses this problem. This unique device can cool the measurement chamber, enabling it to be set at any user-defined temperature from 18 to 42 °C, guaranteeing stable conditions every time, in every lab, across every season, anywhere in the world.

In cell culture applications, confluence assessment is an important quality control parameter that is commonly used to estimate the proportion of adherent cells on a growth surface as an indicator of the cell density inside a well or culture flask. Some cell lines behave differently regarding their growth rate or gene expression depending on the degree of confluence. Consistent determination of cell confluence and estimation of cell numbers are therefore important for reproducible assays and accurate interpretation of experimental data. However, visual cell imaging using microscopes is usually a time-consuming and laborious process, especially when multiple samples are being analyzed. A solution for this problem is automated cell confluence assessment, realized in the Spark® 20M multimode reader.

Cell culture - Application notes

CellSpring’s 3D Bloom microtissue platform is a new technology that shows promise for 3D cell culture. It can be used to make microtissues of virtually any cell type. The rapid assembly of cells within the 3D Bloom scaffold enables the generation of microtissues ready for testing in as little as 30 minutes, compared to hours or days using other methods.

TAP Biosystems (now part of Sartorius) has developed a 3D cell culture technique – RAFT™ (Real Architecture for 3D Tissue) – that allows researchers to culture their choice of cell type, including primary cells, in an in vivo-like environment. This application note documents the automated production of four 96-well RAFT cell culture plates in parallel, using the HCT-116 cancer cell line as an example for a typical tumoroid-forming cell line. It compares cellular viability and response to the anti-cancer compound doxorubicin in RAFT 3D cell cultures and classical 2D cell cultures.

Tecan and TAP Biosystems have developed an automated process for the preparation of RAFT™ cell cultures. Implementation on the Freedom EVO® system, with its modular automation capabilities, increases experimental versatility and analytical flexibility. In this application note, we present cell viability and proliferation data for primary neonatal human dermal fibroblasts obtained over an 11-day culture period after seeding.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. The Alvetex® scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.

Combining Tecan’s Infinite® M200 PRO with InSphero’s organotypic microtissue tumor model provides a scalable system to assess drug sensitivity in complex 3D cell culture models. This allows long-term, tissue-based analyses – such as cell proliferation studies – offering reproducible measurements over time.

This application note demonstrates the potential advantages of automated stem cell culture in biopharmaceutical and cell-based therapy applications, where a consistent quality of cultured cells is essential.

Establishing protocols for automated harvesting of various cell lines from multi-well plates.

Researchers in the Department of Biochemical Engineering, University College London (UCL), UK, have automated stem cell culture on a custom-built Freedom EVO® 100 platform. Benefits compared to manual methods are higher consistency and reproducibility, better protection of cells from contamination, and improved operator safety.

This application note serves as a guide to adapt and establish harvesting protocols for various adherent cell lines growing in Corning® RoboFlask™ robot-friendly cell culture flasks using Tecan’s Freedom EVO® liquid handling platform equipped with a flask handling device (Flask Flipper).

Cell culture - Posters

TAP Biosystems (now part of Sartorius) and Tecan have worked together to develop an automated process that enables researchers to increase throughput, facilitate mixing procedures and enhance reliability while maintaining cell viability, reproducibility, experimental versatility and analytical flexibility.

To simplify the adoption of 3D techniques in drug discovery, this project investigated the automation of a hydrogel-based modular 3D cell culture system using a liquid handling robot. This approach was proven to be absolutely comparable to manually-produced hydrogels.

Oncotest maintains a unique repository of >300 tumor xenograft models representing all major histotypes as well as niche tumors. After evaluating many 3D cell culture platforms, Oncotest has chosen Alvetex® scaffold 96 because of its simplicity, low cost of adoption and flexibility to study tumor cell cytotoxicity, as well as mechanism of drug action at the translational and post-transcriptional levels.

Cloning and transfection - Application notes

The Institute for Research in Immunology and Cancer (IRIC) at the University of Montreal established a reliable, fully automated cloning workflow on a Freedom EVO® workstation. This system performs fully automated multi-fragment DNA assembly, customized plating in ANSI/SLAS-format plates, colony picking and PCR set-up – as well as agar plate preparation – allowing the group to greatly increase the throughput of its experiments.

A Freedom EVO® 150 workstation with a SciRobotics’ Pickolo™ Colony-Picker module proved the ideal solution to perform both fungal selection and the subsequent screening of different culture conditions. The Freedom EVO workstation can easily identify, select and pick fungal colonies based on user-selected criteria, and achieved 100 % microinoculum growth for subsequent experiments due to Pickolo’s unique picking procedures.

The Nucleofector® technology from Lonza has been specifically designed for difficult-to-transfect cell lines and primary cells, including stem cells, T lymphocytes and non-dividing neurons. This application note describes the integration of the Nucleofector into Tecan’s Freedom EVO® liquid handling platform to fully automate the transfection process.

Cloning and transfection - Posters

Using primary cells for RNAi-based applications, such as target identification or validation, requires a highly efficient transfection technology in combination with a reliable and robust automation system. To accomplish these requirements, we integrated the Amaxa™ Nucleofector™ 96-well Shuttle® System into a Tecan Freedom EVO® cell transfection workstation.

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