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Tab 01 / 基于细胞的测定
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iPSC libraries offer a treasure trove of cellular resources, derived from diverse individuals and genetic backgrounds. These libraries hold the key to understanding human development, unravelling the mysteries of diseases, and paving the way for groundbreaking advancements in drug discovery, personalized and regenerative medicine.
概述
细胞水平筛选在药物开发过程十分重要。而自动化有助于加速先导化合物和细胞靶点的分析。一个成功药物筛选项目，其关键点就是运行稳定的细胞水平的实验测试。Tecan提供模块化的解决方案，适用于各种细胞水平的筛选实验，无论是高通量还是低通量，只需要一款仪器就能满足您测试实验开发和日常常规筛选的工作。
主要优点
轻松设置剂量反应实验，和结果分析。
Tecan的专业检测仪器，是自动化模块的理想组化。它通过优化试剂和细胞的准备测试方法，可提供可靠且可重复的细胞活性，毒性及细胞凋亡评估和测试
Spark Cyto 微孔板检测仪是专为活细胞测试实验设计的，可以提供广泛的分析技术：如比色法、荧光法、光度法和细胞成像来帮助您分析细胞的健康状态。基于图像的细胞活性和凋亡研究的预设应用方法，可以即时生成高质量的细胞数据。
您可以使用Tecan的任何一种液体处理工作站，您的剂量反应测试进行梯度稀释，如果您遇到复杂的化合物板的设计和加样，还可以选择我们的D300e 数码加样器。
为了实现工作流程的完全自动化，细胞板可以储存在自动化的、有环境控制的二氧化碳培养箱中，您还可以根据您实验的需求添加设备，如振动筛、洗板机、成像多功能检测器或高内涵检测仪，以及用于储存孔板和耗材的自动化转盘堆栈。
自动化系统能提供更可靠的细胞间的信号通信研究解决方案。
Tecan整合其检测解决方案，提供可靠、完整的基于激酶活性和G蛋白反应的实验自动化工具，用于研究细胞信号通路。同时我们还有层流罩可供集成，可为长时间的细胞反应测试（如表观遗传学研究），提供最佳的细胞培养保护。
Spark Cyto微孔板检测仪将多种检测技术相结合，其中包括TR-FRET，荧光偏振，ALPHA®技术和基于成像的细胞分析学等多种先进技术，用于研究细胞信号通路。
您可以使用Tecan的任何一种液体处理工作站，您的剂量反应测试进行梯度稀释，如果您遇到复杂的化合物板的设计和加样，还可以选择我们的D300e 数码加样器。
为了实现工作流程的完全自动化，细胞板可以储存在自动化的、有环境控制的二氧化碳培养箱中，您还可以根据您实验的需求添加设备，如振动筛、洗板机、成像多功能检测器或高内涵检测仪，以及用于储存孔板和耗材的自动化转盘堆栈。
帮您找出哪些基因在特定细胞事件中被表达。
报告基因分析是学术界和制药企业生命科学研究的重要组成部分。报告蛋白的表达可通过直接检测该蛋白或测量其酶活性来监测。Spark Cyto活细胞微孔板检测仪提供预设的应用方法，采用标准的荧光和发光检测能力以及基于荧光的细胞成像，可以轻松评估转染效率和报告蛋白的活性。
由于这些测试反应的运行时间都比较长，所以合适的细胞保护和培养就至关重要。为了实现工作流程的完全自动化，细胞板可以储存在自动化的、有环境控制的二氧化碳培养箱中，您还可以根据您实验的需求添加设备，如振动模块、洗板机、成像多功能检测器或高内涵检测仪，以及用于储存孔板和耗材的自动化转盘堆栈。
Access to human pluripotent embryonic stem cells is enabling Genea Biocells to pioneer novel therapies to treat a number of neuromuscular diseases. Drawing on almost 30 years of research heritage, the company is using its expertise to model spinal muscular atrophy and facioscapulohumeral muscular dystrophy to identify potential therapies.
The Monash Antibody Technologies Facility (MATF) in Victoria, Australia, has added a new dimension to its high throughput service, offering screening for antibody functionality. This time-consuming phase is crucial for many projects, and can now be outsourced to MATF, where comprehensive and flexible automation completes testing in a fraction of the time.
The new Spark 20M multimode microplate reader offers tailor-made solutions to suit virtually any drug discovery or advanced life science research application. This freely configurable system gives researchers access to new techniques and features intended to enhance and streamline biochemical and cell-based workflows.
The Karlsruhe Institute of Technology has automated protocols for high throughput separation of cells for therapeutic applications on a Freedom EVO® 200 liquid handling platform. The process maintains cell viability and is more rapid and reproducible than manual methods, enabling processing of 96 samples in four hours.
Scientists in the ADME Screening, Enzymology and Automation Group at Roche, Switzerland, have successfully integrated a mosquito® nanoliter liquid handling instrument with a Freedom EVO® platform, enabling accurate, precise pipetting at very low volumes.
Scientists working in the Screening Unit at the Institute for Molecular Pharmacology in Germany are finding the low volume pipetting capability of the MultiChannel Arm™ 384 beneficial for the investigation of siRNAs and small molecule compound screening.
Tecan and pION Inc. have combined pION’s PAMPA Explorer Test System™ with Tecan’s microplate readers for drug permeability screening using the Double-Sink™ parallel artificial membrane permeability assay (PAMPA™).
The German army has automated the determination of AChE activity in whole blood. The determination of cholinesterase status is primarily intended for analyzing patients poisoned with organophosphates (accidental or suicidal poisoning), but it can also be applied after the use of nerve agents in military conflicts or terrorist attacks, as well as in the context of occupational medical examinations.
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Tab 02 / 细胞成像与分析
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概述
Tecan的多功能酶标仪包括细胞成像功能，适用于广泛的细胞检测分析。 高通量酶标仪的集成使得可以直接观察细胞对刺激的反应，而流式细胞术允许分离特定的细胞类型。
Key benefits
从吸光度，荧光和发光测量到精确的细胞计数和基于亮场的细胞成像，发现全系列的酶标仪。
Tecan的酶标仪为您基于细胞的应用提供了广泛的数据分析方法。 Spark系列的高灵活性将所有主要的检测技术结合在一个易于使用的仪器中。
阅读更多内容将MACSQuant®系列仪器集成到Tecan的液体处理平台中，从您基于细胞的测定中收集有价值的数据。
细胞生物学工作流程通常需要使用定义的细胞密度以便正确解释数据。
The MACSQuant flow cytometers from Miltenyi Biotec provide comprehensive multi-parameter flow cytometric analysis using up to three lasers and 10 optical channels. All sample preparation steps – from cell dilution to reagent addition with mixing and shaking – are fully automated, before the microplates are then transferred to the MACSQuant for final analysis.
现在，基于细胞的高通量筛选通常用复杂的光学测定法来补充以研究细胞对小分子，miRNA，抗体或其他化合物等刺激物的反应。
自动化的高通量筛选过程，结合标准化的细胞培养过程，包括孵化，收获，电镀和培养基交换 - 有助于减少样品和用户之间的差异性。
The integration of your choice of high content reader (eg. ArrayScan™ from Thermo Scientific, IN Cell Analyzer from GE Healthcare Life Sciences, Cellavista from SynenTec) makes Tecan’s liquid handling platform a complete solution.
Cell separation has become instrumental in many areas of medical research over the last two decades, and Miltenyi Biotec’s MACS® Technology has become the gold standard technique, having been cited in over 20,000 publications. Combining superparamagnetic microparticles conjugated to specific antibodies with proprietary columns, this technology uses strong magnetic fields to separate specific cell types, allowing retention of both labeled and unlabeled cells for downstream analysis.
自从2月份在SLAS 2015推出以来，Spark®10M已经在整个生命科学界获得了兴趣，全球研究人员开始探索这种令人兴奋的新系统的先进功能，包括DNA定量，微生物学研究和基于细胞测定。
The Karlsruhe Institute of Technology has automated protocols for high throughput separation of cells for therapeutic applications on a Freedom EVO® 200 liquid handling platform. The process maintains cell viability and is more rapid and reproducible than manual methods, enabling processing of 96 samples in four hours.
新的Spark 10M多功能酶标仪旨在为细胞生物学和基因组学客户提供更大的灵活性和更高的生产力。 从微生物学研究和基于细胞的测定到快速的DNA定量，全新的平台提供高级功能的组合并且非常易于使用，以简化您的日常工作。
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Tab 03 / 细胞培养
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概述
从传统的单层培养到复杂的3D细胞培养和高度细腻干细胞的技术，Tecan为您提供适合您应用的工具。 培养基交换，扩展和抗体生产等常规过程都是全自动化的。
Key benefits
CellSpring的3D Bloom生物聚合物平台是一种专有技术，可以使用几乎任何类型的细胞生产3D microtissue。自然衍生的3D Bloom生物聚合物能够使细胞友好的交联，组织细胞形成3D结构，允许细胞-细胞和细胞-基质相互作用，密切模拟组织在体内的行为。Tecan与CellSpring合作，在Freedom Evo平台上开发了将速度和多功能性、一致性和再现性结合起来3D Bloom细胞培养的自动化高通量解决方案。
Tecan和Tap Sartorius已经开发出一种用于制备RAFT 3D细胞培养的自动化流程方案。RAFT 现在正由Lonza Bioscience代理销售。具有专利保护的RAFT系统提供了一个简单、一致性强的和重现性好的96孔板格式的耗材，为细胞提供了一个生物相关的胶原基质环境。该流程方案经过优化，可为很多的细胞生物学应用（包括基于细胞的筛选、成像和生物化学技术）提供快速可靠的无人工干预的流程处理。
Tecan与Reprocell的合作开发了基于Freedom Evo®平台，使用Alvetex Scaffold进行常规3D细胞生物学的自动化解决方案。96孔板格式的Alvetex Scaffold可用于目前使用二维细胞生物学技术的任何实验室。它在多种细胞和分子技术和生化分析中提供了一种极好的体内模拟环境，这都因为细胞显示出增强的细胞功能和反应能力。
Tecan与InSphero合作开发的无Scaffold的3D细胞培养系统，InSphero是仿生药物测试的有机3D微组织球体的主要供应商。细胞可以在96孔Gravityplus™平板的悬浮培养液滴中生长，能够高效可靠地形成完全不与人工支撑基质或表面接触的球体。96孔GravityTrap™检测板确保微组织集中在孔内，提供适当的光学特性和与多功能检测器有良好兼容性（如Infinite®200 Pro）。
提高劳动密集型抗体生产过程的生产力。
产生针对大量抗原的抗体是一项耗时的过程，并且需要许多复杂的步骤，例如细胞融合，杂交瘤克隆选择和细胞扩增。自动化所有或甚至只是最劳动密集型的加工步骤可以显著提高生产力，使得单一设备可以每年生产数千种单克隆抗体。
通过专门的工具和设备自动化主要瓶颈，如培养基交换，细胞电镀，孵化和测定准备。
培养容器
1-1536 孔板，试管，培养皿
自动化
孔板、盖转移处理、试管转移和板倾斜处理
存储
大容量二氧化碳培养箱、工作台培养箱、消耗品存储（转盘、堆站）
细胞图像
细胞融合测算，细胞计算
防污染
带高效过滤器和紫外线防护的无菌环境或二级生物安全柜
自动处理精细的干细胞以获得可重复的结果。
通过将仪器置于无菌环境中，能够完全自动化具有或不具有饲养细胞的多能细胞和分化细胞的生长。综合检测装置跟踪和反应于不期望的自发分化。该系统的灵活性还使您能够吸取微量生长因子，细胞因子和其他添加剂，以及提供大量介质和缓冲液分配。
一种新颖的3D细胞培养平台使科学家能够产生与几乎任何细胞类型的人类组织行为密切相似的微量组织。这种专有技术易于自动化，为高通量应用提供高速度,多功能性，一致性和可重复性。
退行性眼病对世界各地的数百万人造成影响，导致视力逐渐丧失，在某些情况下，甚至完全失明。澳大利亚的研究人员正在使用干细胞技术来研究如何阻止或甚至扭转常见眼睛疾病的影响。
Karlsruhe理工学院拥有在FreedomEVO®200液体处理平台上用于治疗应用的高通量分离细胞的自动化方案。该过程保持细胞活力，并且比手动方法更快速和可重复，使得能够在四小时内处理96个样品。
Tecan和TAP Biosystems通过在FreedomEVO®工作站上自动化TAP的RAFT™细胞培养技术，创建了一个自动化的3D细胞培养筛选平台。该系统使用类似于体内的胶原基质浓度来确保在与天然组织非常相似的环境中的细胞生长和增殖，并增加了自动化液体处理的可靠性和产量。
基于Freiburg的Oncotest有限公司专门从事临床前药理优选法研究，并在FreedomEVO®工作站上成功自动化3D细胞培养，使用了Reinnervate的聚苯乙烯基的Alvetex®Scaffold 96孔板
Tecan和InSphero开发了一种基于Infinite®M200 PRO和InSphero 3D InSight™微量组织技术以分析3D细胞培养的新颖解决方案。该解决方案旨在促进在药物筛选应用中使用3D细胞培养形式，有助于降低早期药物开发的成本。
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Tab 04 / 克隆和转移
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概述
成功的细胞生物学工作流程依赖于生成高质量的克隆。 Tecan提供从脂质转染等标准方法到专门的核转染方法的全自动转染。 随后通过集落采集或细胞分离装置的集成来选择理想的克隆。
主要优势有：
细菌，真菌，藻类，杂交瘤和哺乳动物细胞集落的轻松可靠的采摘。
自动化您的克隆和细胞系开发工作流程中最劳动密集型和容易出错的步骤。专为FreedomEVO®平台而设计，可以将SciRobotics的Pickolo™集落采集器与NAP，PCR设置或蛋白质纯化等上下游实验无缝集成。
主要优势有：
集成全自动的、高通量的培养皿接种解决方案，完成您的克隆和微生物筛选工作流程。
手工涂板十分繁琐，尤其是考虑到制备连续稀释液。将SciRobotics Petriplater™与Tecan的液体处理平台相结合，提供量身定制的解决方案，以加快涂板速度并节省实验人员的手工操作时间。
展示更多在一个平板中设置各种稀释液进行菌落计数：
帮您自动化您费时费力的实验室流程。
从细胞制备和转染混合物到转染板的培养，Tecan为各种细胞类型的转染提供可靠的自动化解决方案。
Spark Cyto活细胞微孔板检测仪提供预设的应用方法，采用标准的荧光和发光检测能力以及基于荧光的细胞成像，可以轻松评估转染效率和报告蛋白的活性。
Tecan的工作站在使用多种方法进行自动转染方面有着很好经验和纪录，包括脂质体、磷酸钙或聚乙烯亚胺（PEI）为基础的转染，或Lonza的Nucleofector™96 Well Shuttle™系统。Tecan的自动化设备还可用于siRNA的筛选。
核酸构建体的产生和表达通常基于标准化试剂盒。帝肯与最著名的套件制造商合作，提供无忧自动化协议。无论您的个人工作流程解决方案需要清洁的工作台、二级生物安全柜、二氧化碳培养箱、保持样品和试剂稳定的冷却或加热设备，还是多模式成像阅读器，帝肯都可以为您提供量身定制的解决方案。
从随机生成的细胞系池中更快地找到最佳克隆。
细胞系发育是任何生物制剂治疗的核心，但是在某种程度上产生稳定的生产细胞系用于研究和生产仍然是随机的。该过程中最关键和不一致的步骤是从随机生成的细胞系池中选择最佳克隆。自动化可以应用于有限稀释电镀，板复制，评估单细胞克隆起源，克隆采摘，使用ELISA的进行表达筛选,蛋白质定量以及一批蛋白质表征方法，以减少开发时间并满足数据和过程文件的法规要求。
Tecan的自动化解决方案可应用与有限的稀释涂板，平板复制，单克隆挑选和分选，通过ELISA的表达筛选，蛋白定量和其他蛋白表征方法学。同时还能缩短开发时间和满足数据和流程的文档记录和监管要求。
发现用于高通量细胞和生物分子分离和分析的独特而全面的解决方案。
Tecan和Miltenyi Biotec已经联手。可以选择在MultiMACS™Cell24 Separator Plus上分离未改变的活细胞, 或MultiMACS M96thermo分离器上以分离病毒和生物分子，如核酸或蛋白质，而不用担心交叉污染。
主要优势有：
全球化的抗菌素耐药性日益严重的问题导致食品生产商面临越来越大的压力，要求他们停止在牲畜饲料中使用预防性抗生素作为添加剂。为了帮助对抗这对动物健康造成的潜在风险，农业生物科学正在利用鸡、猪和牛肠道中存在的微生物群的力量，试图消除病原体的生长。
蒙特利尔大学的研究人员受益于Tecan与SciRobotics的合作，以自动化合成生物学的DNA装配协议。系统生物学和合成生物学研究部门结合Freedom EVO®200与Pickolo™集落采集器和各种其他组件，构建了简化的克隆工作流程，增加了生产量和可靠性。
西班牙能源制造商Repsol依靠两个FreedomEVO®平台和Tecan的350μl嵌套液体处理一次性加样针进行替代能源研究，加长时间的无人值守对此至关重要。
俄罗斯近年来一直是生物技术和生命科学快速增长的地区，粮食和农业是重点关注领域。这是来自实验室的一个快照，它们研究微生物在食品生产中的对比作用，在工作流程中都采用了Tecan设备。
实验室自动化和LIMS专家Mejoran 实验室自动化公司开发了一种用FreedomEVO®150平台进行自动真菌菌落采集的定制方法，该系统具有集成的SciRobotics Pickolo™集落采集器。这种创新的菌落采集方法为下游测定生产一致质量的活性微菌剂，挑选突变体进一步调查所需的时间从一周减少到一天。
石原三洋开发有限公司中央研究院生命科学实验室利用Tecan的FreedomEVO®200工作站开发了一种成功的自动化方案，用于有效的siRNA转染，为小RNA文库的有效高通量筛选铺平了道路。
使用FreedomEVO®系统和SciRobotics的Pickolo™集落采集器的自动化彻底改变了Evogene实验室的基因克隆和集落采集工作流程，从而实现了克隆过程的高效可靠跟踪，并使科学家能够提高克隆的年产量。
Tecan和amaxa 股份有限公司（现在的Lonza）已经将amaxaNucleofector®96孔Shuttle®系统与Tecan FreedomEVO®液体处理工作站成功整合，允许全自动，高通量转染原代细胞和难以转染的细胞系，包括非分裂神经元和T细胞，以及siRNA，DNA和其他底物。
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Discover tailor-made solutions for your cell biology workflow from cell maintenance and cell-based assays to life cell imaging.
Tecan has teamed up with pION Inc. to provide an automated solution for running high throughput drug permeability screening, using the pION Double-Sink™ parallel artificial membrane permeability assays (PAMPA™) and μSOL™ solubility assay. The flexibility of Tecan’s Freedom EVO® workstation and Infinite® M200 PRO microplate reader, combined with Pion’s Gut-Box™ microplate stirrer and PAMPA Evolution™ technology, provides a complete solution for drug candidate screening.
Tecan has created a range of automated ADME screening systems which maximize the efficiency and throughput of these processes. These systems are based on our liquid handling platform for automated cell permeability, compound characterization, in vitro drug metabolism and cell culturing procedures.
Discover a platform that speaks the language of your laboratory, and make your cell-based assays simpler, more efficient… and fluent.
This white paper outlines how the Fluent® Automation Workstation was used to automate cell culture for beating, hiPSC-derived cardiomyocytes. It also describes the use of these cultured cells to screen >3,500 small molecules in a chemically induced hypertrophy disease model, using a validated phenotypic assay.
This application note describes the successful automation of cytotoxicity endpoint assays using mES-D3 and NIH 3T3 cells on the Fluent® Automation Workstation. Researchers at Fraunhofer ITMP ScreeningPort have combined automated cell culture maintenance and homogenous cell viability assays with high content imaging and analysis to optimize the EST and develop a robust, high throughput screening platform to predict negative effects on embryonic development in vivo.
This application note describes the successful automation of an mES-D3 differentiation assay, including embryoid body formation on the Fluent Automation Workstation. In combination with the automated workflow for compound toxicity assessment in mES-D3 and NIH3T3 cells, researchers at Fraunhofer ITMP ScreeningPort have optimized the EST workflow and developed a robust screening platform to predict negative effects on embryo development in vivo.
Fraunhofer IME has adapted the Pluricyte Cardiomyocyte Differentiation Kit protocol for 96-well plates, using the Fluent Automation Workstation to create a screening platform for compound-related cardiotoxic effects.
The data presented in this application note underlines the suitability of the D300e Digital Dispenser for protein-based assays.
The results generated with the D300e Digital Dispenser are consistent with an expertly performed manual process, but with the benefit of significant time savings and the elimination of serial dilution.
Fully automated HDAC assays on the Fluent® Laboratory Automation Solution where triplicates of 10 serially diluted compounds per plate were tested for dose response to selective histone deacetylase inhibition.
The ability to carry out cell-based G-Protein coupled receptor (GPCR) assays in high throughput is a key part of the drug discovery process. Combining a Fluent® Laboratory Automation Solution configured for cell-based assays with the DiscoverX PathHunter® β-Arrestin human and ortholog GPCR assay kits gives users the power to run 384-well plate-based assays in high throughput.
Growth inhibition (GI50) of a set of eight compounds was tested on four different NCI-60 cell lines on a Fluent® Laboratory Automation Solution.
Easy automation of Cisbio’s EPIgeneous™ cell-based assays on a Fluent® Laboratory Automation Solution. In order to discover the optimal assay conditions, four different cell densities of three different cell lines were deployed to determine the level of H3K27Me3 and total H3 under different conditions.
Increasing sample numbers drive many laboratories to look for opportunities to automate and optimize their liquid handling processes. One of the dilemmas that laboratories face when configuring their automated liquid handling systems is the need to balance capacity and functionality against space and budget constraints.
The PAMPA Explorer Test System™ implemented on Tecan´s Infinite® M200 PRO multimode microplate reader comprises a quick, reliable system that accurately models both human jejunal permeability (GIT) and rodent blood-brain barrier (BBB) uptake of candidate drug molecules, based on measurements of permeability across artificial phospholipid membranes.
Streamlining 3D cell culture-based drug response profiling with the Fluent® Automation Workstation, D300 Digital Dispenser, and Spark® Cyto multimode reader.
Cytochrome P450 screening assay automated in a 384-well microplate format for lead generation using three different CYP enzymes.
Easy automation of a biochemical assay on the Fluent® Laboratory Automation Solution to identify coagulation factor IIa inhibitors in a 384-well plate format.
The Spark multimode microplate reader platform off ers solutions to suit virtually any life science research or drug discovery application. It allows researchers to freely confi gure the reader to their current needs, and is fully upgradable to access other techniques and features in the future.
This Technical Note describes the functions and benefits of the Spark multimode microplate reader and cell imaging module, as well as the SparkControl software and its dedicated Cell Counting and Cell Viability Application.
In this technical note, we demonstrate the superior performance of the Spark reader compared to two other multimode readers from different providers. The detection limit for the far red fluorescent dye Alexa Fluor 647 – which has a fluorescence spectrum comparable to Cy5 – was determined, operating each instrument in MCR mode.
Cell counting, viability analysis and determination of size distribution are routinely performed by researchers across multiple disciplines, from standard cell passaging for downstream experiments to using insect cells infected with baculovirus to produce recombinant proteins, or determining the differentiation capacity of stem cells. Tecan’s Spark multimode reader has an integrated, bright field cell imaging module. This patent-pending system enables label-free cell counting, size distribution determination and trypan blue-based cell viability analysis with an easy-to-use, disposable Cell Chip™. In this study, the analysis of cell concentration, cell viability and size distribution for the determination of the optimal protein production rate in recombinant Sf21 cell cultures is presented.
For many cell biology applications – such as the identification and separation of high expression clones or knock-down candidates, and small-scale antibody or protein production – it is important to ensure that cell lines originates from a single cell, i.e. they are monoclonal. This can be a major bottleneck, especially for academic laboratories, as dedicated automated microscopy systems are both very expensive and complex to operate, meaning verification of monoclonality is often performed by manual microscopy.
Tecan’s Spark multimode microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cell confluence, thereby boosting automation of cell-based studies.
Cell migration is a central process in the development and maintenance of multicellular organisms, with tissue formation during embryonic development, wound healing and immune responses all requiring the orchestrated movement and assembly of cells.
Until now, it has only been possible to heat multimode readers using temperature control, not to set them at ambient temperature or lower. This can cause problems for temperature-sensitive experiments, such as enzymatic assays, affecting the reproducibility of results over time. In addition, the experimental precision can be decreased by the plate heating up during measurements, even over just a few minutes. Tecan’s Te-Cool™ module for the Spark® platform, the first air conditioning system for a multimode reader, addresses this problem. This unique device can cool the measurement chamber, enabling it to be set at any user-defined temperature from 18 to 42 °C, guaranteeing stable conditions every time, in every lab, across every season, anywhere in the world.
In cell culture applications, confluence assessment is an important quality control parameter that is commonly used to estimate the proportion of adherent cells on a growth surface as an indicator of the cell density inside a well or culture flask. Some cell lines behave differently regarding their growth rate or gene expression depending on the degree of confluence. Consistent determination of cell confluence and estimation of cell numbers are therefore important for reproducible assays and accurate interpretation of experimental data. However, visual cell imaging using microscopes is usually a time-consuming and laborious process, especially when multiple samples are being analyzed. A solution for this problem is automated cell confluence assessment, realized in the Spark® 20M multimode reader.
There has been growing interest in the use of in vitro three-dimensional (3D) cell culture in cancer research and tissue biology over the last couple of decades. 3D spheroids are more physiologically relevant compared to conventional 2D cell culture, making them more suitable for in vitro efficacy testing and analysis of drug penetration, bridging the gap between traditional monolayer cultures and animal testing. Furthermore, 3D cell models are promising tools in personalized medicine, as tumor spheroids can be obtained from most tumors, and can be used to screen drugs to find effective patient-specific therapies. Alternatively, healthy multicellular spheroids are potential organ building blocks to restore lost organ functions in regenerative medicine applications.
Automated cell seeding, medium exchange and incubation of 3t3-l1 cells using the fluent automation workstation and thermo scientific’s cytomat™ 2 c450-lin automated incubator
This application note compares accuracy and reproducibility between manual and automated seeding of adherent cells in 96-well microplates using the Fluent Automation Workstation. We show how the Fluent platform can successfully increase productivity while achieving high quality results.
This application note describes optimized protocols for the maintenance and reprogramming of patient-derived fibroblast cells into induced pluripotent stem cells (iPSCs) and their differentiation into retinal cells using the Freedom EVO® platform.
This application note describes the automated production and growth of single spheroids in ultra-low attachment (ULA) Corning 96-well spheroid microplates on a Fluent Automation Workstation, using a Multiple Channel Arm™ (MCA) for cell seeding and medium exchange.
CellSpring’s 3D Bloom microtissue platform is a new technology that shows promise for 3D cell culture. It can be used to make microtissues of virtually any cell type. The rapid assembly of cells within the 3D Bloom scaffold enables the generation of microtissues ready for testing in as little as 30 minutes, compared to hours or days using other methods.
TAP Biosystems (now part of Sartorius) has developed a 3D cell culture technique – RAFT™ (Real Architecture for 3D Tissue) – that allows researchers to culture their choice of cell type, including primary cells, in an in vivo-like environment. This application note documents the automated production of four 96-well RAFT cell culture plates in parallel, using the HCT-116 cancer cell line as an example for a typical tumoroid-forming cell line. It compares cellular viability and response to the anti-cancer compound doxorubicin in RAFT 3D cell cultures and classical 2D cell cultures.
Tecan and TAP Biosystems have developed an automated process for the preparation of RAFT™ cell cultures. Implementation on the Freedom EVO® system, with its modular automation capabilities, increases experimental versatility and analytical flexibility. In this application note, we present cell viability and proliferation data for primary neonatal human dermal fibroblasts obtained over an 11-day culture period after seeding.
Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. The Alvetex® scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
Combining Tecan’s Infinite® M200 PRO with InSphero’s organotypic microtissue tumor model provides a scalable system to assess drug sensitivity in complex 3D cell culture models. This allows long-term, tissue-based analyses – such as cell proliferation studies – offering reproducible measurements over time.
This application note demonstrates the potential advantages of automated stem cell culture in biopharmaceutical and cell-based therapy applications, where a consistent quality of cultured cells is essential.
Establishing protocols for automated harvesting of various cell lines from multi-well plates.
Researchers in the Department of Biochemical Engineering, University College London (UCL), UK, have automated stem cell culture on a custom-built Freedom EVO® 100 platform. Benefits compared to manual methods are higher consistency and reproducibility, better protection of cells from contamination, and improved operator safety.
This application note serves as a guide to adapt and establish harvesting protocols for various adherent cell lines growing in Corning® RoboFlask™ robot-friendly cell culture flasks using Tecan’s Freedom EVO® liquid handling platform equipped with a flask handling device (Flask Flipper).
TAP Biosystems (now part of Sartorius) and Tecan have worked together to develop an automated process that enables researchers to increase throughput, facilitate mixing procedures and enhance reliability while maintaining cell viability, reproducibility, experimental versatility and analytical flexibility.
To simplify the adoption of 3D techniques in drug discovery, this project investigated the automation of a hydrogel-based modular 3D cell culture system using a liquid handling robot. This approach was proven to be absolutely comparable to manually-produced hydrogels.
Oncotest maintains a unique repository of >300 tumor xenograft models representing all major histotypes as well as niche tumors. After evaluating many 3D cell culture platforms, Oncotest has chosen Alvetex® scaffold 96 because of its simplicity, low cost of adoption and flexibility to study tumor cell cytotoxicity, as well as mechanism of drug action at the translational and post-transcriptional levels.
The Institute for Research in Immunology and Cancer (IRIC) at the University of Montreal established a reliable, fully automated cloning workflow on a Freedom EVO® workstation. This system performs fully automated multi-fragment DNA assembly, customized plating in ANSI/SLAS-format plates, colony picking and PCR set-up – as well as agar plate preparation – allowing the group to greatly increase the throughput of its experiments.
A Freedom EVO® 150 workstation with a SciRobotics’ Pickolo™ Colony-Picker module proved the ideal solution to perform both fungal selection and the subsequent screening of different culture conditions. The Freedom EVO workstation can easily identify, select and pick fungal colonies based on user-selected criteria, and achieved 100 % microinoculum growth for subsequent experiments due to Pickolo’s unique picking procedures.
The Nucleofector® technology from Lonza has been specifically designed for difficult-to-transfect cell lines and primary cells, including stem cells, T lymphocytes and non-dividing neurons. This application note describes the integration of the Nucleofector into Tecan’s Freedom EVO® liquid handling platform to fully automate the transfection process.
Using primary cells for RNAi-based applications, such as target identification or validation, requires a highly efficient transfection technology in combination with a reliable and robust automation system. To accomplish these requirements, we integrated the Amaxa™ Nucleofector™ 96-well Shuttle® System into a Tecan Freedom EVO® cell transfection workstation.
仅供研究使用。