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核酸纯化

所有基因组学工作流程中的关键流程

从各种类型的样本中提取核酸是所有基因组工作流程的一部分。

Tecan正在全球范围内与Zymo Research、MACHEREY-NAGEL、Promega和Omega-Biotek等合作伙伴合作,开发自动DNA/RNA提取和纯化解决方案,以满足您的不同样本类型与通量的需求。




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Tab 01 / DreamPrep NAP工作站
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DreamPrep® NAP工作站

DreamPrep NAP工作站汇集了基于磁珠法的提取工作流程所需的硬件、软件和耗材。可以为每批1-96个样本提供了一个随时可用的处理方案,并整合了定量和均一化功能。

Tecan与各种核酸提取供应商合作,为不同的样本类型与应用开发预编程的和优化的方案。

Click here to open the list of available scripts

* 请复制这些方法,以便根据你的预期用途和实验室,对其进行定制以适应和验证你的工作流程。

Frida-Reader_DreamPrep-NAP_720x484

DreamPrep与定量/均一化

DreamPrep NAP提供以下综合定量和均一化的选项:

DreamPrep NAP与我们的试剂合作伙伴

帝肯与核酸纯化试剂制造商紧密合作,为核酸的自动化提取提供高质量的解决方案。




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Tab 02 / 磁珠法核酸提取
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自动化核酸提取方法

基于磁珠的

基于磁珠的纯化方案很适合自动化,可以灵活处理不同样本数,并避免了批量处理的需求。可以调整方法以增加或减少起始样本量,使您能够提取实验所需的正确数量的DNA或RNA。

 

Click here to open the list of available scripts

高通量自动化

Fluent®基因组学

Fluent的开创性设计为基因组工作流程提供了更高的通量和更快的速度。这种创新的解决方案提供了卓越的精度,出色的吞吐量和更长的无人值守时间。

  • 每次最多可以纯化四个96孔板
  • 可选择扫描96位得原始样本管和洗脱管的二维码
阅读更多内容

可扩展的自动化

Freedom EVO® 100/150 NAP 工作站

无论您是在寻找自动化处理1-96个样本的灵活8通道机械臂还是想要加速纯化流程的96通道加样机械臂,帝肯有一系列经过充分测试的实验协议和配置,以满足您的需求,包括可选的扩展工作流程。

  • 原始样本管的条码扫描和样本跟踪
  • 酶标仪用于定量和均一化
  • TouchTools™界面

智能自动化

优化的Freedom EVO 75配置

如果您的实验室空间有限,想要免动手磁珠提取高纯度核酸,这款价格实惠的台式工作站是理想的解决方案。

  • 从全血、人或动物组织、培养细胞和细菌中提取基因组DNA的现成方案
  • 专用的TouchTools™界面
下载彩页以了解更多信息




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Tab 03 / 真空法核酸提取
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自动化核酸提取方法

基于真空的

固相核酸纯化(NAP)流程允许使用整合的抽真空装置进行快速处理,可使用96孔板或8连管。

 

Click here to open the list of available scripts

智能自动化

优化的Freedom EVO 75配置

如果您的实验室空间有限,想要免动手磁珠提取高纯度核酸,这款价格实惠的台式工作站是理想的解决方案。

  • 从全血、人或动物组织、培养细胞和细菌中提取基因组DNA的现成方案
  • 专用的TouchTools™界面
下载彩页以了解更多信息

可扩展的自动化

无论您是在寻找自动化处理1-96个样本的灵活8通道机械臂还是想要加速纯化流程的96通道加样机械臂,帝肯有一系列经过充分测试的实验协议和配置,以满足您的需求,包括可选的扩展工作流程。




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Tab 04 / High throughput extraction
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High-throughput workflows

Tecan addresses the high-throughput (HT) demand for extraction workflows with its Fluent Automation Workstation, offering flexible and fully automated solutions for a wide range of sample types.

This platform supports magnetic bead-based extraction and can be configured with advanced modules to streamline and scale up laboratory processes facilitating:

  • Walkaway automation for increased efficiency and reduced hands-on time
  • High sample throughput ( variable formats up to 96 or more samples per batch)
  • Compatibility with multiple HT extraction kits
  • Seamless integration with downstream applications such as NGS, qPCR, and dPCR

 

NAP_high_trhoughput_v01_720x520

 

NAP_high_trhoughput_v02_720x520

 

Tecan’s Fluent 780 Automation Workstation, integrated with two KingFisher Presto units, provides a fully automated, high-throughput solution for nucleic acid extraction using Thermo Fisher’s MagMAX kits.

This setup enables:

  • Walkaway processing of up to four plates at once in 24- or 96-well formats.
  • Advanced liquid handling including efficient large sample volume handling.
  • Barcode tracking, and user-friendly software combine with magnetic bead-based purification for reliable, high-quality nucleic acid recovery

In Partnership with:

Thermo-Fisher-Scientific_v2




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Tab 05 / Qualified NAP protocols
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Check our list of available protocols for NAP.


Please read the method definitions in the bottom section.

Veya NAP DreamPrep NAP Fluent
Supplier Method Kit Veya®
NAP
DreamPrep® NAP Fluent®
NAP
Macherey-Nagel | NAP Mag | NucleoMag DNA Microbiome | | DreamPrep NAP | green | Vendor | Macherey-Nagel
Bacterial and viral DNA/RNA
NAP Mag NucleoMag DNA Microbiome   V    
Macherey-Nagel | NAP Mag | NucleoMag Pathogen​ | | DreamPrep NAP | green | Vendor | Macherey-Nagel
Bacterial and viral DNA/RNA
NAP Mag NucleoMag Pathogen​   V    
Macherey-Nagel | NAP Mag | NucleoMag VET | | DreamPrep NAP | green | Vendor | Macherey-Nagel
Bacterial and viral DNA/RNA
NAP Mag NucleoMag VET   V    
Macherey-Nagel | NAP Mag | NucleoMag Food | | DreamPrep NAP | blue | Macherey-Nagel
DNA extraction
NAP Mag NucleoMag Food      
Macherey-Nagel | NAP Mag | NucleoMag Tissue | | DreamPrep NAP | green | Vendor | Macherey-Nagel
DNA extraction
NAP Mag NucleoMag Tissue   V    
Macherey-Nagel | NAP Mag | NucleoMag® 96 Plant | | DreamPrep NAP | green | Vendor | Macherey-Nagel
DNA extraction
NAP Mag NucleoMag® 96 Plant   V    
Macherey-Nagel | NAP Vac | NucleoSpin 96 Plasmid | | DreamPrep NAP | green | Vendor | Macherey-Nagel
Plasmid DNA
NAP Vac NucleoSpin 96 Plasmid   V    
Macherey-Nagel | NAP Vac | NucleoSpin 96 Plasmid Transfection-Grade | | DreamPrep NAP | green | Vendor | Macherey-Nagel
Plasmid DNA
NAP Vac NucleoSpin 96 Plasmid Transfection-Grade   V    
Omega Bio-tek | NAP Mag | Mag-Bind Universal Pathogen | | Fluent NAP | green | Vendor | Omega Bio-tek
Bacterial and viral DNA/RNA
NAP Mag Mag-Bind Universal Pathogen     V  
Omega Bio-tek | NAP Mag | Mag-Bind Viral DNA/RNA 96 | | DreamPrep NAP | blue | Fluent NAP | green | Vendor | Omega Bio-tek
Bacterial and viral DNA/RNA
NAP Mag Mag-Bind Viral DNA/RNA 96     V  
Omega Bio-tek | NAP Mag | Mag-Bind cfDNA | | Fluent NAP | green | Vendor | Omega Bio-tek
Cell-free DNA
NAP Mag Mag-Bind cfDNA     V  
Omega Bio-tek | NAP Mag | Mag-Bind® Blood & Tissue DNA HDQ 96 | | DreamPrep NAP | green | Vendor | Fluent NAP | green | Vendor | Omega Bio-tek
DNA extraction
NAP Mag Mag-Bind® Blood & Tissue DNA HDQ 96   V   V  
Omega Bio-tek | NAP Mag | Mag-Bind TotalPure NGS | | Fluent NAP | green | Vendor | Omega Bio-tek
Fragmented DNA
NAP Mag Mag-Bind TotalPure NGS     V  
Promega | NAP Mag | Maxwell® HT Viral TNA, Custom | | DreamPrep NAP | green | Vendor | Fluent NAP | pink | Vendor | Promega
Bacterial and viral DNA/RNA
NAP Mag Maxwell® HT Viral TNA, Custom   V   V  
Qiagen | NAP Mag | QIAprep&® Viral RNA UM Kit | | Fluent NAP | green | Vendor | Qiagen
Bacterial and viral DNA/RNA
NAP Mag QIAprep&® Viral RNA UM Kit     V  
Thermo Fisher Scientific | NAP Mag | MagMax™ Viral/Pathogen Nucleic Acid Isolation​ | | Fluent NAP | pink | Vendor | Thermo Fisher Scientific
Bacterial and viral DNA/RNA
NAP Mag MagMax™ Viral/Pathogen Nucleic Acid Isolation​     V  
Zymo Research | NAP Mag | Quick-DNA/RNA™ Viral MagBead | | Veya NAP | blue | Tecan | DreamPrep NAP | green | Vendor | Fluent NAP | green | Vendor | Zymo Research
Bacterial and viral DNA/RNA
NAP Mag Quick-DNA/RNA™ Viral MagBead T   V   V  
Zymo Research | NAP Mag | ZymoBIOMICS™ 96 MagBead DNA | | DreamPrep NAP | green | Vendor | Fluent NAP | green | Tecan | Zymo Research
Bacterial and viral DNA/RNA
NAP Mag ZymoBIOMICS™ 96 MagBead DNA   V   T  
Zymo Research | NAP Mag | MAGicBead™ cfDNA Isolation | | DreamPrep NAP | green | Tecan | Zymo Research
Cell-free DNA
NAP Mag MAGicBead™ cfDNA Isolation   T    
Zymo Research | NAP Mag | Quick-DNA/RNA™ MagBead | | DreamPrep NAP | pink | Vendor | Fluent NAP | blue | Vendor | Zymo Research
DNA extraction
NAP Mag Quick-DNA/RNA™ MagBead   V   V  
Zymo Research | NAP Mag | Quick-DNA™ MagBead Plus | | Veya NAP | green | Tecan | DreamPrep NAP | green | Vendor | Zymo Research
DNA extraction
NAP Mag Quick-DNA™ MagBead Plus T   V    
Zymo Research | NAP Mag | EZ-96 DNA Methylation MagPrep | | DreamPrep NAP | green | Vendor | Zymo Research
Epigenetics
NAP Mag EZ-96 DNA Methylation MagPrep   V    
Zymo Research | NAP Mag | Quick-DNA™ HMW MagBead | | DreamPrep NAP | yellow | Zymo Research
HMW DNA
NAP Mag Quick-DNA™ HMW MagBead      
Zymo Research | NAP Mag | Quick-DNA/RNA MagBead | | DreamPrep NAP | pink | Vendor | Fluent NAP | blue | Vendor | Zymo Research
RNA isolation
NAP Mag Quick-DNA/RNA MagBead   V   V  
Zymo Research | NAP Mag | Quick-RNA MagBead | | DreamPrep NAP | pink | Vendor | Fluent NAP | blue | Vendor | Zymo Research
RNA isolation
NAP Mag Quick-RNA MagBead   V   V  
 

Method available:

Automated method developed according to the kit vendor’s published protocol. Protocol was tested using water and/or reagents with Tecan instruments.

 

Method qualification in progress:

Automated method developed according to the kit vendor’s published protocol. It is currently being tested with reagents and commercial samples by the vendor.

 

Method qualified: V T C

Automated method developed according to the kit vendor’s published protocol. Method was qualified using kit vendor’s guidelines, reagents and commercial samples. Libraries prepared with these methods meet the kit vendor’s specifications and/or perform comparably to those prepared manually. Sequenced libraries were assessed either by the vendor (V vendor qualified), by Tecan (T Tecan qualified) or by the customer (C customer qualified).

 

Method custom developed:

Automated method developed according to specific customer need. Libraries tested by the customer.




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Tab 06 / 文献
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