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您的研发伙伴:Spark®

多功能微孔板检测仪

您可以根据不同的应用方向从 四款现有配置 中的一款选择以解决您的研究难题。

可自由配置的平台还可以让您自由的对自己的Spark检测仪进行个性化配置。

最后Spark还可以在将来任何时间进行模块添加和升级。




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Tab 01 / 概述
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Four configurations for today. Ready for tomorrow.

基因组学和蛋白组学

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微生物学

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细胞实验

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药物研发

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Spark典型功能

  • 光吸收 –包括紫外/可见光光谱扫描
  • 荧光顶底读 – 包括荧光光谱扫描(3D)
  • 时间分辨荧光 (TRF) – 包括TRF光谱扫描
  • 荧光共振能量转移(FRET)
  • 时间分辨荧光共振能量转移(TR-FRET)
  • 荧光偏振 (FP)
  • 化学发光 – 辉光,闪光,多色发光和高灵敏发光光谱扫描
  • AlphaSreen®、AlphaLISA® 和 AlphaPlex®
  • 自动实时细胞明场成像—细胞计数和细胞汇合度
  • 光吸收立式比色杯
  • NanoQuant微量检测板
  • 温度控制(室温+3°C – 42°C)
  • 带加热和搅拌的自动液体加样器
  • CO2 & O2 气体控制模块蒸发保护
  • 挥发保护(湿度盒)
  • Te-Cool™ 制冷 ( 主动温度控制范围 18-42 ℃ )
  • 内置自动开盖
  • QC 工具 (IQ/OQ 服务)
  • ELISA
  • 微量 DNA/RNA 定量
  • 核酸标记效率
  • 蛋白定量
  • 报告基因
  • HTRF® 均相时间分辨荧光
  • Transcreener®
  • DLR® 双报告基因
  • BRET – 包括 NanoBRET®
  • 细胞计数和活力
  • 汇合度评价
  • 细胞迁移和伤愈
  • Tecan Connect™ 移動應用監控儀器

您是否准备探索自己的Spark应用极限?

光吸收

超快速、超精确、功能丰富

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荧光

无与伦比的灵活性,可适应任何试验或预算。

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化学发光

在最广泛的应用中具有无与伦比的灵敏度。

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细胞计数和成像

以经济的成像方式获得更高的数据置信度。

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Tab 02 / 技术
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高速光栅检测器

在五秒内完成完整的吸收光谱扫描

Spark独有、申请专利中的的高速单色仪可为吸光度测量树立新的速度和精确性标杆。

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DNA spectrum of a 50 μg/ml sample after blank reduction; a 2 μl sample volume was measured in Tecan’s NanoQuant Plate.

波长精度与扫描速度的完美结合

Spark的高速单光栅的创新设计在5秒内提供了200-1000纳米的吸收光谱,使用的阶跃尺寸仅为1纳米,有效地消除了样品蒸发的风险。光谱记录以无与伦比的波长精度和再现性(<=0.3nm)进行,为每个样品生成一致的数据。

这种速度、准确度和重现性的结合使Spark成为小体积核酸定量和纯度检测的首选仪器。

The image shows OD linearity from 0 to 4 OD measured at 260 nm.

核酸定量分析的优越表现

Spark卓越的波长精度和再现性,结合帝肯Nanoquant Plate™的独特设计,保证DNA检测下限为<1 ng/μl。

A260/280或A260/230中良好的波长比精度对于DNA/RNA纯度检测至关重要,它们的精度是确保数据的重现性,但市场上大多数检测器系统都没有特定参数设定。

Spark的超快速的光谱记录使您能够在不存在蒸发风险的情况下对NanoQuant 中的所有16个样品进行全光谱波长扫描。而这个风险对于其他的基于单色仪的吸收光检测仪是一个已知的风险点。

NanoQuant微量检测板

无需校准的小量核酸定量

已获专利的NanoQuant微量检测板是首款专为小量核酸多功能酶标仪吸光度分析而设计的工具。其适用于广泛的应用,如DNA或RNA定量、核酸纯度评估和标记效率测定。

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无需校准量的定量板

NanoQuant 板可以同时检测多达16个样本,样本体积仅为2uL。可以使用传统的单道或八道移液器完成加样。每一个石英微点都是当作一个单独的透镜使用—这样保证了每个样本的光的通过性——并可以被快速、容易地清洁以最大的限度去降低交叉污染的风险。关上板盖就可以生成16个固定光路的0.5mm的石英微型比色皿,用于230、260和280 nm的测量。

为了方便使用和优化数据质量,NanoQuant板是市场上唯一一种100%无校准的低容量板,在保持可靠性能的同时尽量减少维护时间。这种创新的工具是在你的实验室中进行灵敏度高、可靠和容易的DNA/RNA定量的先决条件。

除了吸光度测量外,NanoQuant板还可用于检测小体积样品中的荧光信号。只需用加入的用于荧光测试的2微升样品(例如Quant IT™Picogreen®dsDNA Assay)到NanoQuant板上,然后就可以使用Spark检测荧光信号。

NanoQuant 和 Spark的结合可以为核酸定量提供终极方案。

预定义方法=自动样本分析

SparkControl™软件为核酸定量提供了一种预先配置的“一键式”方法,只需按一下按钮即可快速、简单和可靠地得出结果。

The detection limit of ssDNA is specified at less than 1 ng/µl using the NanoQuant Plate in combination with Spark’s High Speed Monochromators.

荧光 – Fusion 光路

革命性设计带来出色灵敏度和灵活性

Spark的Fusion光路是首个在单次荧光测量中,将单色仪的灵活度与滤光片的灵敏度结合在一起的解决方案。

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一款卓越的多功能检测器

Spark可以配备滤光片,Tecan最先进的QuadX Monochromators™双光路系统。不同于其它多功能酶标仪,这个设计可以允许客户独立的在滤光片和光栅之间进行选择。

这一独特的功能可用于所有基于荧光的检测模式,包括标准的顶部和底部测量、TRF、FRET、TR-FRET和FP,提供非常低的检测限值。SparkControl软件提供了对系统的轻松控制,可以方便地选择滤光片和光栅。

Closing the gap between flexibility and sensitivity

Spark’s unique ability to combine the flexibility of monochromators with the sensitivity of filters is especially relevant for fluorescence emission or excitation scans. For example, using a filter on the excitation side gives higher instrument sensitivity, significantly improving peak quality.

The image shows a comparison of excitation and emission scans for standard fluorophore label (A), Alexa Fluor® 555 (B) and europium (C), revealing that using monochromators on both sides only detects ~25 % of the signal compared to using filters on the fixed wavelength side. This allows Spark’s Fusion Optics to dramatically increase the peak quality of the fluorescence spectra, even for very low signals.

Key features for fluorescence

  • Automated Z-focusing – optimal top and bottom focusing in every well, independent of plate type and format
  • Flexible bandwidth selection – from 5 to 50 nm for an optimal balance between specificity and sensitivity
  • Multiple dichroic mirrors – to maximize instrument sensitivity in combination with filters or monochromators
  • Optimal Read function – for bottom reading fluorescence, guaranteeing signal recording from all cells in the well, ideal for detection of GFP signals
  • Well scanning – high density well scanning generates a low resolution fluorescence image of the well
  • Spectra Viewer – software tool for improving excitation and emission settings of your assay
  • 3D scanning – accelerates assay development by providing simultaneous excitation and emission scans, helping to quickly and easily identify changes in the spectral properties of fluorescent probes or characterize unknown samples

发光光路

高灵敏度设计

Spark的发光模块可为 96孔、384孔和1,536孔的微光、闪烁和多色发光应用提供更高的灵敏度。前沿的发光光路设计,可以在保持灵敏度的同时记录光谱,让您获得出色的检测性能,同时又可为未来的发光应用未雨绸缪。

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优化光学=最佳结果

Optimized optics = optimal results

Spark采用针对每种板格式(96、384和1536孔)优化的发光纤维,确保孔之间的最小信号串扰。该光路通过一个创新的滤光片轮引导,该滤光片由多个衰减滤光片组成——将高信号减少多达1000倍——以及38个用户可选择的彩色滤光片,几乎可以实现任何多色发光测量。产生的信号由光电倍增管(PMT)检测,该光电倍增管以其最灵敏的模式工作:光子计数。

这种光学元件的独特组合确保了弱发光信号的最大灵敏度,并允许高信号的衰减。其检测范围高达10^9位于行业领先水平,同时还有对辉光和闪光有极低的检测下限。

Emission spectra of Renilla (RR) and firefly (FF) luciferases recorded with the Spark

用多色发光优化你的实验

大多数孔板检测器采用单光栅荧光发射器进行发光扫描,这将导致由于衰减导致的不可接受的灵敏度下降和较差的结果。

为了克服这一限制,Spark使用了一种基于19对短通和长通滤光片的独特滤波技术。只需点击几下,您就可以为多色发光测量或光谱扫描选择所需的波长和带宽。这使得Spark成为了一个面向未来的多重分析检测系统(如Chroma-Glo™) 、BRET应用(包括NanoBRET™)和发光检测器。

Alpha 酶标仪

Alpha实验技术的完美性能需要高质量的仪器。Spark将一个高功率激光光源与一个创新的滤光片和专用的光子计数PMT相结合,用于超灵敏Alpha实验。

Spark配备了独特的红外温度传感器,能够在测量过程中进行每一个孔的温度校正,克服了众所周知的Alpha实验的挑战。这都得力于Spark的Te Cool™模块,用于控制和提供稳定的温度,甚至低于环境温度,让您完全控制这个关键的实验影响因素。

这些功能确保Spark为AlphaScreen®、AlphaLISA®和AlphaPlex®实验提供无与伦比的性能,以及潜在的未来Alpha应用。

自动化细胞成像

实现细胞计数、活性分析和活细胞成像

在多功能酶标仪之中直接内置简单、高性价比的细胞成像功能。Spark细胞成像模块包括一个LED 明场发光装置、一个4x物镜、一个CMOS 130万像素摄像机和一个牢固的激光自动对焦系统,可提供堪比自动化显微镜的性能。对于希望进入这一激动人心的应用领域的用户,该系统可提供经济的解决方案。

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Cell counting and viability

Cell counting and cell viability assessment of trypsinized cells is a daily task in any cell culture lab. Spark’s cell imaging module offers automated label-free cell counting and trypan blue-based cell viability analysis with an easy-to-use, disposable Cell Chip. Each chip has two sample chambers which can accommodate 10 μl of cell suspension, and four Cell Chips can be loaded onto the Cell Chip adapter, allowing eight samples to be analyzed in less than two minutes.

This user-friendly solution offers: 

  • High throughput, label-free cell counting, even at low cell concentrations
  • One-click live/dead analysis
  • High precision replicate processing for multiple samples
  • Fast and simple mean value calculations, greater accuracy and more precise data

Histogram view to enable adjustment of the analysis based on cell size for the study of sub-populations, eg. for cell swelling assessments

Live cell imaging and confluence

In general, you don’t know how many cells have adhered to the bottom of a microplate well, or even grown, over a certain period of time. Determination of cell confluence and estimation of cell numbers are therefore important for reproducible assay results and interpretation of experimental data.

With Spark’s cell imaging module, you can automatically assess the precise confluence – from 10-90 % –  giving you a new level of data quality in your assays.

Key applications:

  • Visual quality control of cells in microplates – an automated microscope in your reader
  • Label-free cell proliferation – detect cell growth in real time, without manual interference
  • Better assay consistency – always start your assay at the same confluence level, increasing the reproducibility of your cell-based assays
  • Signal normalization – use confluence data to normalize your cell-based signals to the cell mass in your well
  • Quality control – prove the optical quality of your microplate using confluence assessments
  • Wound healing studies– perform semi-automated cell migration and wound healing assays with comparable performance to a dedicated imaging device
  • Single cell cloning – combine automated image acquisition with semi-automated image analysis to verify monoclonality

活细胞工具包

简单的完整环境控制

作为细胞生物学家,您每天的生活就是忙于时刻保持细胞“快乐”。 通过自动化的工作流程,您可以腾出自己被夺走的时间,又能让细胞“快乐”生长。Spark将自动化培养箱、酶标仪及细胞成像功能整合在一起,为您自己及您所在的实验室提供了更多新的可能。

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自动化孵育器

Spark配备了精确的温度控制,使您的微孔板均匀加热。为了确保温度分布均匀,微孔板室与光学元件热隔离,确保稳定精确的温度调节。

Spark通过将CO2/O2控制,还增加另一层的环境控制参数。集成气体控制模块(GCM™)提供从0.04%到10%(体积)的二氧化碳调节,和从0.1%到21%(体积)的氧气调节,为您的细胞创造理想的环境。在长期的动力学研究中,二氧化碳的调节维持了细胞的健康,而氧气的控制使你能够培养微需氧生物或模拟生理条件的正确环境。

对于生产良好的细菌或酵母生长曲线也需要有效的振荡。基于多年的经验和在微孔板中培养细胞系的知识,Spark也专门为微生物的长期振动和孵化进行了优化设计。

易上手的SparkControl软件可以直接控制这些功能,为您的活细胞分析提供最佳条件。

湿度控制-防止边缘效应

95%或更高水平的湿度调节是保持细胞活力和生长良好的另一个关键因素,在长期分析过程中,减少蒸发对于保持恒定浓度至关重要。Spark的 Humidity Cassette 是专门为SBS格式的孔板设计用来减少蒸发,且经济有效的解决方案。该专利工具包括一个填充装置,和配合我们独特的盖子升降机的专用盖子,这都可以用来提供被动的湿度控制。只需在填充装置中添加ddH2O,就可以保持湿润的空气。

板盖升降装置

Spark为您提供了一种新型的自动化系统—集成的盖子开关功能。这种功能创造了新的工作流程可能性,降低了样品污染的风险,并帮助您为长期动力学分析建立了理想的环境。无论您是想在不需要人工干预的情况下分配化合物,还是在不包含蒸发保护的情况下保持最佳环境条件,Spark都是完成这项任务的最佳选择。

为科学家设计的软件

SparkControl将自动化的可以进行动力学调节的长期动力学实验、远程操作和开放动力学等高级功能结合起来,为复杂的实验提供了一种无需手工操作的解决方案。

许多活细胞动力学实验需要特定的操作,例如,一旦达到某个信号水平或OD值,就需要添加化合物。而配有手动控制的多功能酶标仪会使这个实验变得十分繁琐或容易出错。SparkControl的动态调节功能,在无盖或有盖的微板中都能允许您在控制孔中达到预定信号后,对系统进行编程,以自动执行所需的操作,适用于几乎任何您可以想到的分析工作流程。

SparkControl的远程控制功能允许您从实验室外监控或操作仪器。这使您无需坐在Spark前数小时,即可进行完整的系统控制;只要您连接到互联网,就可以连接到您的实验。

多模式检测器经常被不同的用户用于多个应用程序。长期的活细胞动力学分析可以占用该检测器一整天,限制整个实验室的。SparkControl提供了一个有效的解决方案:开放式动力学。此功能允许您暂停和恢复动力学实验,以便在长期研究中执行其他测量。这为其他用户提供了使用的可能性,消除了共享实验室设备的一个主要瓶颈,同时又不影响您有价值的研究。

Te-Cool – 真正的温度稳定性

整体温度控制 - 即便在低于环境温度的情况下

一致的温度是确保获得可靠结果的先决条件。Spark是首款内置冷却功能的多功能酶标仪,将整体温度控制在18到42 °C之间。

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不是所以的生物学反应都在37°C

并不是所有的生物学反应都发生在37°C。例如斑马鱼幼虫需要25°C,昆虫细胞(Sf9)需要22°C。常见的多功能检测器只能加热孔板,并通常会有高于室温2~5°C的偏移。这种传统的方法就丢失了灵活性,而且实验的条件也不是最优的,因此还会导致产生不可靠或不可用的数据。这种功能的缺乏也限制了你研究某些生物体或应用的能力。

Exemplary luminescent kinase assay, where the activity of a glow luciferase was measured at different temperatures. The luminescent signal increased approximately 10 % per 1 °C increase in temperature.

温度对发光的影响

温度对使用非哺乳动物生物的酶进行生化分析起着至关重要的作用。例如,生物发光分析系统的最佳温度为20到25°C之间。这些分析测试通常对温度敏感,即使温度升高1°C,发光输出也会增加10%。因此,无法在这个临界范围内稳定控制温度的光度计或多功能检测器将产生不可重现的数据。 Spark是唯一一个可以减轻温度对发光分析实验影响的检测器,为您的有价值的研究提供更高质量的数据

快速冷却

支持多用户的检测仪就需要多种的实验条件。微孔板检测器的一个主要瓶颈,就是需要等待仪器到达所需的温度。加热测量室很容易,但是如果是需要冷却呢?

一般情况下,一个被动降温的检测仪通常需要60分钟才能使37°C冷却到室温,这个时间会耗费您宝贵的时间还会打乱您繁忙的日程安排。想象一下,在多用户的实验室里,光光是降低冷却的时间就能有效避免项目延迟和效率的降低。

Te-Cool 模块的主动冷却的微孔板板室,有效降低等待时间。为您的实验提供37 °C到 22 °C快速稳定的温度环境。

Luminescent signal over time in a kinetic measurement. During a measurement time of 6 min, the luminescent signal increases due to heating of the liquid in the wells).

Prioritize your valuable resources

Researchers in every lab are constantly being pushed and pulled to make the next discovery on limited assets. Whether the constraint is driven by budget or time, we have all felt the burden.

Spark brings together a unique set of features intended to ease this strain and create new workflow possibilities for your laboratory, reducing hands-on time while producing high quality data for each and every sample.

Whether you are looking to design long-term experiments that work around your schedule, attempting to shrink sample volumes to reduce costs, or trying gain access to multiple instruments on a tight budget, Spark gives you control over your priorities. 

Eliminate data variability at the source

As scientists, we consider ourselves data-driven. Whether your research is centered in academia or industry, your next discovery will rely on your data. The ability to generate reliable, reproducible and high quality data should be a prerequisite for all laboratory instruments.

Every time you run an experiment you invariably introduce some level of error that impacts your results. Variability is hard to avoid, whether it is transferring your cell-based assay between instruments or manually assessing confluence. Whether the errors are human subjectivity, environmental factors or contamination, you are influencing your results. Completely eliminating this variability isn’t realistic, so you are forced to make concessions. But isn’t your data too important to compromise?

Spark is designed to address almost every detail of the most demanding assays and applications, delivering technologies that seamlessly work together to allow you to generate accurate and consistent data, each and every day.




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Tab 03 / 软件
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SparkControl™ 适应您的检测需要

SparkControl擅长处理常规或复杂工作流程。无论您需要定量某种核酸,还是要以特定的吸光值自动化加样, SparkControl 均能胜任,帮助您腾出更多的时间进行自己的研究。

可用性处于中心地位

真正的简便性

一键式应用程序可简化您的大多数检测,让您以前所未有的速度从样品获得结果。SparkControl采用图标驱动、“拖放式”操作,适于任何技术水平的用户。

轻松实现复杂应用

SparkControl基于工作流程的界面,允许您即刻访问应用程序并享受轻松的操作。该软件允许用户轻松将现有实验方案转化为可随时运行或编辑的操作方法。

视频

Open Kinetic

不用再担心注册表单。

Open Kinetic 允许您暂停和恢复动力学读书。在长期实验或具有长读取间隔的实验的情况下,此功能允许其他用户在动力学测量之间还能执行其他的应用。

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Live Viewer

最近一次拍摄的影像。SparkControl让Spark变成了一个显微镜。

Live Viewer允许用户在最佳生理条件下实时监控细胞。

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内置荧光团文库

包含精确光谱的荧光团列表,让您快速可视化,轻松选择激发和发射波长。

集成了超过60个荧光团的文库,可以方便地、精确地、可视化地、选择发射和激发波长。

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Highlights

  • 一键式应用程序
  • 环境控制
  • 最佳的读取效果
  • 自动化Z-聚集
  • 高清孔扫描
  • 高分辨率 3D 扫描
  • 扩展的动态范围
  • 动力学检测保护
  • Magellan

高分辨率 3D 扫描

加速您的检测开发

高分辨率3D扫描可同时进行激发和发射扫描,使您能够快速识别荧光探针光谱特性的变化,以及表征未知荧光样品。

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扩展的动态范围

检测极低信号

在测量过程中自动调整增益设置意味着您不必牺牲灵敏度,允许您能检测非常低的信号。

SparkControl为您整理所有数据,并将所有结果关联并显示在一个数据表中。

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自动的Z轴聚焦

更高的荧光灵敏度

能显著提高您结果的质量,有效的增强顶读的荧光强度和荧光偏振的灵敏度。

自动Z聚焦调整能找到了每个微孔板孔中最佳信空白比的焦点,无论板型、孔形或样品体积。

这一独特的特性使您可以方便地设置检测器,以便在不同的分析参数下获得最佳性能。

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一键式应用程序

以前所未有的速度从样品获得结果

我们致力于开发快速、简单的仪器操作,并通过SparkControl的一键式应用程序呈现给您。

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高清孔扫描

完整的细胞群图像

即使是不均匀的细胞层,我们也能从每个孔都获得更精确的信号。

高清晰度井扫描给你一个细胞分布的定性图像,有效地给你一个完整的细胞群图片。

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环境控制

一键调节条件

与Spark独特的环境控制模块(包括GCM™和Te-Cool™模块)相结合,SparkControl可以方便地动态调整参数。

您可以完全控制运行期间的环境条件,包括温度(甚至低于环境温度)和检测仪内的二氧化碳和氧气水平。

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最佳的读数荧光测量

优化的底部荧光读取

SparkControl具有特殊的为旨在改进细胞学测量的优化读取(OR)功能。该功能通过对每个孔上排列的空间分离点进行多次测量,提高了FI底部读数的灵敏度。

这使您在测量微孔板中的贴壁细胞时,孔间和孔内都能有很好的重现性,因而能够得到非常低的cv值。

Optimal Read fluorescence measurements - Diagram

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动力学检测保护

为您的检测提供安全防护

自动增益调节可以保护您的动力学分析,以避免荧光测量进入饱和状态。

然后具有不同增益设置的测量值会自动关联,从而可以对整个数据集进行评估。

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SparkControl Magellan™

增强您的数据分析能力

SparkControl Magellan是一个数据分析包,为您提供了多种检测模式的数据分析工具。

有了SparkControl Magellan,您可以体验到针对各种微孔板的应用的功能性,简便性以及安全性的性能提升。

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Tecan Connect™ mobile app

Stay connected with your experiment wherever you are.

Use the Tecan Connect mobile app to monitor instrument status and alert you when user interactions are required.

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Spark选配件

带加热和搅拌的自动液体加样器

综合加样器使您具备了极佳的加样能力,非常适合高灵敏度应用,如闪光。独特的加热和搅拌功能,减少了可预知的风险。

Spark-StackTM微孔板堆栈

Spark-Stack是一款结构紧凑的自动化微孔板堆栈,目的在于整合到Spark酶标仪当中。它可以为实验提供极其可靠的无人值守处理,每次运行可容纳多达50个微孔板。

QC 工具 (IQ/OQ 服务)

整个寿命周期内的可靠通量。Tecan的质量控制组合包可帮助您的Tecan酶标仪高效、高性价比地符合所有监管要求。

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挥发保护(湿度盒)

内置蒸发保护功能可以增强活细胞动力学检测,最大限度降低边缘效应,获得更具可重复性、更可靠的数据。

具有专利技术的湿度盒,能减少了标准微孔板中的蒸发,使长期的动力学研究无需使用到专用和昂贵的微孔板类型——只需使用当前经过验证的板即可。其他优点还包括:

  • 有效减少实验过程中的蒸发
  • 兼容现有孔板-不需要昂贵的特制板
  • 更好的数据- 结果一致性好
  • 减少边缘效应-长期动力学实验的理想选择
  • 稳定的环境条件- 更好的细胞活力
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为细胞计数而生的Cell Chips™

Tecan创新的Cell Chips(细胞芯片)最大限度减少了样品制剂的准备工作,带来更大的应用灵活性,为悬浮细胞的计数开启了新机会。

通过内置的细胞计数器,Spark可以分析各种细胞大小和类型,在30秒内提供全自动、无标签的细胞计数和分析。

  • 准确、可重复的细胞计数,具有更高灵敏度的灵活区域选择
  • 一键式分析细胞数量、大小分布和生存能力
  • 可导出图像以进行再次确认
  • 即使在低浓度下也可提供高精度的细胞计数
  • 一次性的Cell Chips可以自动重复处理多个样本
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NanoQuant Plate™

NanoQuant Plate允许同时定量和分析低至 2 µl 至多16种核酸或蛋白质样品。NanoQuant Plate的独特设计,每份样品选择1/4,消除了校准需要,并可保证一致的性能。




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Application Guide

LEARN – SELECT – MEASURE

Application Notes

A lot of research is devoted to the search for new compounds with bioactivity against bacteria. However, it is important to distinguish between antimicrobial activity and antibiofilm activity. Antimicrobial activity affects bacterial growth, and reduced growth can simultaneously result in a decrease in biofilm production. Antibiofilm activity only affects the formation of biofilms. Therefore, the use of antimicrobial compounds at concentrations that are not bactericidal against all susceptible bacteria often leads to the emergence of resistant strains. In contrast, antibiofilm compounds do not affect bacterial growth, and so do not generate the same opportunities for resistance to develop. This Application Note describes the SIMBA – SIMultaneous detection of antiMicrobial and anti-Biofilm Activity – method using the Spark multimode reader to generate OD600 growth curves for S. Infantis in a 96-well microplate over a 20- hour period, allowing the parallel determination of antimicrobial and antibiofilm activities of selected cold water fungal extracts.

Environmental pathogens are microorganisms that inhabit our surroundings, living outside of a human host for a large portion of their lifecycle. Many of these micro-organisms cause severe pathogenic conditions in plants and are often the reason behind significant crop failures that in turn lead to economic damage. Environmental pathogens can also cause diseases in humans and, in some cases, lead to severe infections that are difficult to treat. There is therefore a need for accurate methods to study these pathogens, and a common way to do that is through turbidimetric measurements. The aim of the current study was to investigate how bacterial growth is affected by temperature, using two different environmental pathogens – the rod-shaped Gram-negative Pseudomonas syringae and Erwinia amylovora.

The Optimal Read function for fluorescence bottom measurements is designed to enable uniform well illumination during excitation while and whole-well emission for 6-to 384-well plates. Performing multiple measurements on spatially separated spots arrayed across each well ensures that even heterogeneous cell layers can be effectively and reproducibly analyzed. This application note shows that the Optimal Read function produces the most reliable results for the analysis of adherent cell types, giving the highest well-to-well uniformity and best sensitivity for cell-based applications.

Cell counting, viability analysis and determination of size distribution are routinely performed by researchers across multiple disciplines, from standard cell passaging for downstream experiments to using insect cells infected with baculovirus to produce recombinant proteins, or determining the differentiation capacity of stem cells. Tecan’s Spark multimode reader has an integrated, bright field cell imaging module. This patent-pending system enables label-free cell counting, size distribution determination and trypan blue-based cell viability analysis with an easy-to-use, disposable Cell Chip™. In this study, the analysis of cell concentration, cell viability and size distribution for the determination of the optimal protein production rate in recombinant Sf21 cell cultures is presented.

HTRF is a time-resolved fluorescence resonance energy transfer (TR-FRET)-based assay system from Perkin Elmer cisbio, and is popular for the analysis of various molecular interactions and binding studies. HTRF assays place high sensitivity demands on multimode readers. The Spark offers a number of features to meet these demands and to ensure optimal assay performance, including Tecan’s unique Fusion Optics which combine the sensitivity of filters and the flexibility of monochromators (MCRs) in one instrument. In addition to entirely filter- or MCR-based measurements, a combination of the two can be used simultaneously within one measurement. Automated Z-position optimization and an integrated dichroic mirror that is perfectly suited to HTRF measurements further increase the Spark’s outstanding sensitivity. This application note describes the validation of the Spark reader, equipped with standard fluorescence optics, for HTRF measurements.

This application note describes the successful implementation of AlphaScreen on the Spark multimode reader, as well as the performance of the instrument with regards to measurement sensitivity, uniformity and typical read times.

This application note describes the implementation of the AlphaScreen technology on the Spark multimode reader and the detection of tyrosine kinase activity using the AlphaScreen P-Tyr-100 Assay Kit.

This application note describes the implementation of the AlphaLISA technology on the Spark multimode reader and its use for the detection of human immunoglobulin G (IgG) using the AlphaLISA IgG Assay Kit.

Tecan’s Spark multimode microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cel lconfluence, thereby boosting automation of cell-based studies.

Keep control of crucial experimental conditions with the first air conditioning system for a multimode reader – enabling stable measurement chamber temperature down to 18°C.

Assaying cells in an appropriate window of time after a compound-­mediated cytotoxic event can be extremely challenging. This note describes the automation for kinetic monitoring of cytotoxicity and viability using three Promega assays for viability, cytotoxicity and apoptosis assessment with the Spark multimode reader.

Cell migration is a central process in the development and maintenance of multicellular organisms, with tissue formation during embryonic development, wound healing and immune responses all requiring the orchestrated movement and assembly of cells.

Tecan’s Spark multimode microplate reader was used for the real-time analysis of human stem cell proliferation and differentiation, using established assays for cell viability, proliferation and apoptosis assessment in a reliable and convenient manner.

Tecan’s Spark multimode microplate reader was used to identify metabolic changes during the differentiation of human mesenchymal stem cells and to assess and quantify cell viability, cytotoxicity and apoptosis, using several well-established and reliable cell-based assays developed by PromoCell.

For many cell biology applications – such as the identification and separation of high expression clones or knock-down candidates, and small-scale antibody or protein production – it is important to ensure that cell lines originates from a single cell, i.e. they are monoclonal. This can be a major bottleneck, especially for academic laboratories, as dedicated automated microscopy systems are both very expensive and complex to operate, meaning verification of monoclonality is often performed by manual microscopy.

Homogeneous Time-Resolved Fluorescence (HTRF) is a time-resolved fluorescence resonance energy transfer (TR-FRET) assay system developed by Cisbio Bioassays, and offers a reliable technique for high throughput screening (HTS) and the analysis of molecular interactions. The assay is based on energy transfer between a long-lived europium or terbium cryptate donor and a variety of red or green acceptors, and combines the advantages of FRET and time-resolved fluorescence (TRF).

This application note summarizes the performance of the Spark reader with enhanced fluorescence optics for HTRF assays. All available optics configurations of the Spark’s Fusion Optics system meet the requirements for HTRF certification and are HTRF-compatible.

Technical Notes

Fast and reliable nucleic acid quantification and purity checks are important daily routine applications for many laboratories, driven by the widespread use of next generation sequencing and other high throughput nucleic acid analysis techniques. Many methods require rapid, accurate analysis of small sample volumes to keep pace with downstream processes.This technical note shows the optimized Spark measurement settings, as well as comparative results, for the commonly used fluorescence-based DNA quantification assays evaluated in this study.

This technical note describes a method to perform UV[1]based protein quantification using the Spark multimode reader and the low volume NanoQuant Plate with the one-click SparkControl application. It shows comparative data for UV absorbance-based protein quantification in microplates and the gold standard for dye-based methods, the Pierce™ BCA assay. The study results can be used as a guideline to help scientists choose the right method for their specific application.

Evaporation has a huge impact on the performance of both biochemical and cell-based assays, changing reagent, component and metabolite concentrations to alter reaction kinetics and cell physiology. It is therefore crucial to keep the consistency throughout the whole plate to allow comparison of data between wells during life sciences experiments. This technical note highlights the benefits of using the Tecan’s Humidity Cassette in combination with the Spark. The Humidity Cassette can minimize the impact of edge effects in microplates under certain conditions. It provides better uniformity and delivers more reliable data for long-term studies.

Spark’s fluorescence module is available with a choice of filters, monochromators or Fusion Optics – a novel combination of both monochromators and filters. The Fusion Optics module combines the flexibility of monochromators with the sensitivity of filters in a single device, and even within individual measurements. In addition to this enormous flexibility, users can choose either a standard or an enhanced fluorescence module for their Spark multimode reader. The standard fluorescence module is tailored to basic applications, with an exceptionally good performance in the green wavelength range. The enhanced optics module is designed for higher throughput applications in 384- and 1,536-well plates, and offers better performance in the red and blue wavelength range, as well as for high throughput screening (HTS) assays, including TR-FRET and FP.This technical note describes the differences in the sensitivity-to-speed ratio of the standard and enhanced modules in 96- and 384-well plates for measurements using fluorescein (green) and Alexa Fluor® 555 (red).

Monitoring growth of prokaryotic cells and yeast is an important tool in life sciences, as well as in the food industry. This technical note demonstrates the Spark reader’s suitability for the generation of growth curves in the commonly used 24- and 96-well plate formats for three different, well-established bacterial strains, Escherichia coli, Listeria innocua and Staphylococcus capitis. It describes the established methods to generate OD 600 growth curves of all three bacterial strains in 24- and 96-well plates over a period of 8 hours. The influence of important instrument settings, such as shaking, are considered and discussed.

This technical note outlines the use of the Spark reader in combination with the ValitaTITER assay for the quantification of IgG. It offers a comparison of the use of the instrument’s standard and enhanced fluorescence optics, and discusses the optimization of flash number to produce high quality, reproducible data while minimizing read times.

This technical note outlines the use of the Spark reader in combination with the ValitaTITER assay for the quantification of IgG. It offers a comparison of the use of the instrument’s standard and enhanced fluorescence optics, and discusses the optimization of flash number to produce high quality, reproducible data while minimizing read times.

Spark-Stack is a compact, versatile and field-upgradeable microplate stacker for the Spark® multimode reader. It offers walkaway automation and enhances the productivity in the lab by helping to automate typical workflow steps – including plate loading, unloading and restacking of 6-1536-well microplates. Spark-Stack can be equipped with dark covers to protect light-sensitive assays inside the plate magazines, such as Alpha technology-based assays and fluorophore-transfected cells.

The one-click Live Viewer software application turns the Spark microplate reader into a microscope. It enables easy and straightforward visual quality checks of cells in microplate wells or in Tecan's proprietary Cell Chip™, allowing researchers to automatically acquire and instantly view images of their cell samples immediately before the measurement is started.

The Spark reader is equipped with Tecan's unique Fusion Optics, which combine the benefits of filters and MCRs in a single multimode reader. The Spark can be equipped with filters, Quad4 Monochromators or a combination of both using the ingenious Spark Fusion Optics.

The new Spark multimode microplate reader is equipped with patent-pending High Speed Monochromators (HSM) enabling highly accurate, reproducible and ultra-fast absorbance measurements. This unique optical system offers an enhanced measurement range from 200 to 1,000 nm, ensuring optimal performance across the full range, particularly for absorbance measurements in the deep UV range, such as A260/A230 nucleic acid purity checks. The Spark reader is also suitable for measurements of low volume samples in both absorbance and fluorescence modes using the patented NanoQuant plate.

With the Spark‘s luminescence module, users can perform high sensitivity flash and glow luminescence measurements. For optimized signal detection of very high and low signals within one experiment, optical density (OD) filters help to attenuate strong signals in the range from OD1 to OD3, thereby avoiding ‘over’ readings. This enables users to detect luminescence signals with a dynamic range of more than 9 orders of magnitude.Multicolor luminescence assays with up to five different luciferases, as well as luminescence spectral scanning (eg. of luminescent proteins) can be easily set - using an innovative filter-based approach, users can select any wavelength between 398 nm and 653 nm, with a step size of 15 nm and a 25 nm bandwidth.

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the maximum instrument sensitivity of the Spark multimode reader for flash and glow luminescence.

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the best possible detection limit for standard fluorophore label in fluorescence intensity top reading mode (FI top).

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the best possible detection limit of europium in time-resolved fluorescence intensity reading.

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the best possible detection limit for standard fluorophore label in FI bottom mode.

In this technical note, we demonstrate the superior performance of the Spark reader compared to two other multimode readers from different providers. The detection limit for the far red fluorescent dye Alexa Fluor 647 – which has a fluorescence spectrum comparable to Cy5 – was determined, operating each instrument in MCR mode.

This technical note describes the implementation of the DLR Assay System on Tecan’s next generation high-end multimode reader platform Spark. It can perform high sensitivity flash and glow luminescence measurements with a dynamic range of more than nine orders of magnitude. In addition, its enhanced luminescence module can measure multicolor assays with up to five different luciferases, as well as offering spectral scanning of luminescent substances.

This Technical Note describes the functions and benefits of the Spark multimode microplate reader and cell imaging module, as well as the SparkControl software and its dedicated Cell Counting and Cell Viability Application.

This technical note compares the performance of the Spark’s cell counting module with (i) manual cell counting using C-Chip™ disposable hemocytometers and a standard microscope, (ii) a comparably priced, imaging-based cell counting device (Device A) and (iii) a high performance cell imager with counting capabilities (Device B).

The Transcreener® ADP2 TR-FRET Red Assay is a competitive immunoassay for ADP with a far-red, time-resolved fluorescence-resonance-energy-transfer (TR-FRET) read-out. It is intended for the detection adenosine diphosphate (ADP) production by any kinase or ATPase, and has been developed specifically for high-throughput screening applications. The Spark was tested for its capacity to measure the Transcreener ADP2 TR-FRET Red Assay using the four different combinations of the Fusion Optics.

The Transcreener ADP2 FP assay is a competitive FP assay based on the detection of ADP and is therefore compatible with any enzyme class that produces ADP, including protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases and glutamine synthetase. This technical note describes the instrument settings for the Transcreener ADP2 FP assay on Tecan’s Spark multimode microplate reader.

The Host-Microbiota Interactions Lab at the Wellcome Sanger Institute uses high throughput anaerobic microbiology, genomic and metagenomic approaches to explore the relationship between humans and their microbiome – the community of microorganisms that live on and in us. HMIL has developed techniques to culture strict anaerobic gut bacteria at scale and built an extensive culture collection for phenotypic profiling. To phenotypically screen this culture collection, HMIL uses a Spark multimode reader equipped with an integrated stacker module – the Spark-Stack™ – for automated and high throughput plate processing, which is housed in a Whitley A135 HEPA Anaerobic Workstation. This allows the generation of bacterial growth curves cultured in multiple microplates under stringent anaerobic conditions, i.e. at 0 % O2 using a dedicated and pre-optimized gas mix (80 % N2 , 10 % CO2 and 10 % H2 ). Here, we describe accurate profiling of the growth kinetics of strict anaerobic gut bacteria.

Posters

Tecan’s Spark multimode microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cell confluence, thereby boosting automation of cell-based studies.

Keep control of crucial experimental conditions with the first air conditioning system for a multimode reader – enabling stable measurement chamber temperature down to 18°C.

Cell-based assays are very popular in life sciences research, as they represent an in vitro system which offers results comparable to in vivo systems. However, this improved biological relevance is counterbalanced by the complexity of working with a living organism. Cell-based assays therefore require special precautions and care when planning and conducting experiments

Brochure

Spark 多功能微孔板检测平台,可为几乎所有药物开发和前沿生命科学研究提供量身定制的解决方案。研究人员可根据当前需求在此平台上自由配置功能模块,并可通过升级以获取未来所需的其它技术和特点。

Spark-Stack™ plate stacker for walkaway automation of Tecan Spark® readers Enhance the productivity of your lab with this compact, reliable and field-upgradeable plate stacker. Spark-Stack takes care of typical process steps, such as loading, unloading and re-stacking of non-lidded 6- to 1,536-well plates, and provides a throughput of up to 50 plates per run.




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Tab 06 / 网络研讨会视频
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面向未来的自动化智能多功能微孔板成像检测仪

Tecan推出的Spark®多功能微孔板检测仪,提供量身定制的解决方案,几乎适合任何药物发现或高级生命科学研究应用。其可自由配置的系统使研究人员能够使用最新的技术和功能,帮助研究人员增强和简化生化和细胞学实验的工作流程。

该仪器的核心是Spark独特的融合光路和功能强大的超高频氙气闪光灯,可与您选择的高性能检测模块相结合,确保与您的工作流程完美匹配。

可选模块包括增强荧光模块,旨在提高灵敏度和提高高通量筛选的速度,全新开发的Te-Cool™冷却模块,该模块能允许将测量室温度设置为低于环境室温,从而能获得更准确和可靠的结果。

由于增加了新的自动细胞成像和汇流功能,Spark的细胞处理能力也得到了很高的提升。这使用户可以在测量室内,监测和培养微孔板内的细胞,并能定义分析开始/结束,或注入基质的汇流点。有了这些功能能更好的优化实验,同时也提升了无人值守的时间。有了以上可选模块的支持,1到1536孔板的读取能力,这使得Spark不仅能在今天而且在未来,都能为您的实验室提高更高效的工作效率。

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网络研讨会视频

无人值守的情况下观察细胞毒性、活细胞和死细胞

了解自己的细胞分析工作流程中的细胞活性和细胞毒性检测如何实现简化和自动化 。本网络研讨会焦点为,使用Promega 细胞健康检测和Tecan Spark 多模酶标仪评估细胞毒性、活性细胞和死细胞情况,包括多天的细胞动力学(或实时)分析。

通过评估细胞迁移和细胞活性,提高伤口愈合检测可信度

应用科学家Katrin Flatscher博士和Tecan检测市场经理Michael Fejtl博士将介绍Spark®多模酶标仪成像功能在检测细胞迁移方面的应用。

自动化检测人类细胞分化、增殖及代谢活动

讨论组成员Rüdiger Arnold博士、Katrin Flatscher博士和主持人 Michael Fejtl的这次长谈,将重点关注如何采用PromoCell开发的细胞检测方法及Tecan Spark酶标仪进行的人干细胞分化实时分析,以实现对细胞活性、增殖或死细胞情况快速、方便、灵敏、可重复地评估和定量。

与实验中的热战斗- 面对温度波动造成的扭曲结果

Promega的Gediminas Vidugiris博士和Tecan的Katrin Flatscher博士讨论了许多生化分析的温度敏感性以及实验室中的温度波动如何扭曲测量结果。他们讨论了如何在整个实验工作流程中使用Spark中的Te-Cool™主动冷却功能实现稳定的温度条件。根据基于细胞的生物发光和生物荧光分析的实验数据,通过评估细胞活力、代谢、死亡和信号传导,证明了主动温度控制的好处。

应用视频

探索多模酶标仪内的管盖升降功能

Spark让您可以无需手动干预,使用试剂加热仪和带盖微孔板提升效率。在长时间动力学运行期间,可以通过内置的板盖升降装置,自动去除酶标仪内部的微孔板盖,进行成分加样。

通过“Spectra Viewer”优化您的检测读取

Spark Control内的一种软件工具可帮助您优化所用荧光团的激发和发射设置。

一键式细胞计数和活性检测

Spark细胞染色模块采用易用的一次性Cell Chip™芯片。实现自动化、无需标记的细胞计数和台盼蓝染色法细胞活性分析。

通过“动力学调节”实现对动力学运行完全控制

Spark Control 内的 “动力学调节”软件功能支持自动化设置操作,例如,在指定的对照孔收到某一信号时,启动另一检测模式。

如何在5秒内获得完整的吸收光谱

Spark高速单色仪的创新设计,让您可以在短短5秒内获得200-1000 nm的吸收光谱,且最小增量不过1 nm。

如何通过几次鼠标点击记录高质量的发光光谱

多色发光光路的创新设计,使您可以在保持仪器灵敏度的情况下对发光信号进行光谱记录。

如何将Spark变成一台显微镜

SparkControl把Spark变成了一台显微镜。通过一键式实时浏览应用程序, 即可用4x放大率查看和捕获Cell Chip 或微量滴定板上指定位置的图像。在开始检测前,即可再查看一下您的反应体系。

自动化伤口愈合和细胞迁移

通过Spark reader,对人胆囊癌细胞进行30多小时的Radius™ 96孔细胞迁移检测(5%CO2,37°C,保湿卡盒),弥补了这一空白。

自动化活细胞成像

在Spark酶标仪上,进行50小时内的表皮样癌细胞自动化活细胞成像或汇合度评估(5%CO2,37°C,保湿卡盒)

阅读Spark宣传册

宣传册

Spark多功能酶标仪提供了几乎适合所有生命科学研究和药物研发应用的解决方案

阅读Spark宣传册
参见技术参数

技术参数

Spark 仪器规格及技术参数

参见技术参数

仅供研究使用。