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Spark®多功能酶标仪

专注细节、探索视野、启迪发现

Spark®多功能酶标仪是您的研究合作伙伴 – 致力于满足您今天及未来的需求。

它可以轻松驾驭常规或复杂的检测,并具有模块化可升级设计,,从而实现与您现有工作流程无缝衔接,并创建全新的检测工作成为可能。

其独特的技术和软件功能,可确保无论现在还是将来,总有一款Spark配置能够提升您实验室的生产效率。

Configured for today. Ready for tomorrow.

Spark典型功能

  • 光吸收 –包括紫外/可见光光谱扫描
  • 荧光顶底读 – 包括荧光光谱扫描(3D)
  • 时间分辨荧光 (TRF) – 包括TRF光谱扫描
  • 荧光共振能量转移(FRET)
  • 时间分辨荧光共振能量转移(TR-FRET)
  • 荧光偏振 (FP)
  • 化学发光 – 辉光,闪光,多色发光和高灵敏发光光谱扫描
  • AlphaSreen®、AlphaLISA® 和 AlphaPlex®
  • 自动实时细胞明场成像—细胞计数和细胞汇合度
  • 光吸收立式比色杯
  • NanoQuant微量检测板
  • 温度控制(室温+3°C – 42°C)
  • 带加热和搅拌的自动液体加样器
  • CO2 & O2 气体控制模块蒸发保护
  • 挥发保护(湿度盒)
  • Te-Cool™ 制冷 ( 主动温度控制范围 18-42 ℃ )
  • 内置自动开盖
  • QC 工具 (IQ/OQ 服务)
  • Connect™微孔板堆栈
  • ELISA
  • 微量 DNA/RNA 定量
  • 核酸标记效率
  • 蛋白定量
  • 报告基因
  • HTRF® 均相时间分辨荧光
  • Transcreener®
  • DLR® 双报告基因
  • BRET – 包括 NanoBRET®
  • 细胞计数和活力
  • 汇合度评价
  • 细胞迁移和伤愈

您是否准备探索自己的Spark应用极限?

光吸收

超快速、超精确、功能丰富

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荧光

无与伦比的灵活性,可适应任何试验或预算。

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化学发光

在最广泛的应用中具有无与伦比的灵敏度。

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细胞计数和成像

以经济的成像方式获得更高的数据置信度。

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高速单色仪

在五秒内完成完整的吸收光谱扫描

Spark独有、申请专利中的的高速单色仪可为吸光度测量树立新的速度和精确性标杆。

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DNA spectrum of a 50 μg/ml sample after blank reduction; a 2 μl sample volume was measured in Tecan’s NanoQuant Plate.

Unmatched combination of wavelength accuracy and scanning speed

The innovative design of Spark’s High Speed Monochromator delivers an absorbance spectrum between 200-1,000 nm in just five seconds using a step size of just 1 nm, effectively eliminating the risk of sample evaporation. Spectral recording is performed with unsurpassed wavelength accuracy and reproducibility (<=0.3 nm), generating consistent data for every sample.

This combination of speed, accuracy and reproducibility makes Spark the instrument of choice for low volume nucleic acid quantification and purity checks.

The image shows OD linearity from 0 to 4 OD measured at 260 nm.

Excellence in Nucleic acid analysis

Spark’s exceptional wavelength accuracy and reproducibility, combined with the unique design of Tecan’s NanoQuant Plate™, result in a DNA detection limit of <1 ng/µl.

A good wavelength ratio accuracy in A260/280 or A260/230  is crucial for DNA/RNA purity checks, ensuring repeatable and reproducible data, but this parameter is not specified for most systems on the market.

Spark’s ultra-fast spectral recording enables you to perform whole spectrum wavelength scans for all 16 samples in the NanoQuant Plate without the risk of evaporation, which is a well-known issue for other monochromator-based absorbance instruments.

NanoQuant微量检测板

无需校准的小量核酸定量

已获专利的NanoQuant微量检测板是首款专为小量核酸多功能酶标仪吸光度分析而设计的工具。其适用于广泛的应用,如DNA或RNA定量、核酸纯度评估和标记效率测定。

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Calibration-free quantification

The NanoQuant Plate can simultaneously measure up to 16 samples, with sample volumes of as little as 2 µl easily applied to individual quartz micro-spots using conventional single or eight-channel pipettes. Each quartz micro-spot is shaped to function as an individual lens – guaranteeing the best possible light transmission through the sample – and can be quickly and easily cleaned to minimize the risk of cross-contamination. Closing the plate lid effectively creates 16 quartz micro-cuvettes with a fixed path length of 0.5 mm – perfect for 230, 260 and 280 nm measurements.

For convenience and optimal data quality, the NanoQuant Plate is the only low volume plate on the market that is 100 % calibration free, maintaining reliable performance while minimizing demands on your time. This innovative tool truly is a prerequisite for sensitive, reliable and easy DNA/RNA quantification in your laboratory.

In addition to absorbance measurements, the NanoQuant Plate can be used to detect fluorescence signals in low volume samples. Simply pipette 2 µl samples from your fluorescence assay – for example, the Quant-iT™ PicoGreen® dsDNA assay – onto the NanoQuant Plate and use Spark  to detect the fluorescence signal.

Combining the NanoQuant Plate and Spark offers the ultimate solution for nucleic acid quantification.

Predefined methods = automated sample analysis

SparkControl™ software offers a preconfigured, ‘one-click’ method for nucleic acid quantification, giving you quick, easy and reliable results at the touch of a button.

The detection limit of ssDNA is specified at less than 1 ng/µl using the NanoQuant Plate in combination with Spark’s High Speed Monochromators.

荧光 – Fusion 光路

革命性设计带来出色灵敏度和灵活性

Spark的Fusion光路是首个在单次荧光测量中,将单色仪的灵活度与滤光片的灵敏度结合在一起的解决方案。

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A leap ahead of hybrid readers

Spark can be equipped with filters, Tecan’s state-of-the-art QuadX Monochromators™ double monochromator system or a combination of both. Unlike hybrid multimode readers, this concept allows users to independently choose between filters and monochromators for both excitation and emission.

This unique feature is available for all fluorescence-based detection modes – including standard top and bottom measurements, TRF, FRET, TR-FRET and FP –delivering very low detection limits and unlocking new possibilities. SparkControl software provides effortless control of the system, enabling easy selection of filters or monochromators on the fly.

发光光路

高灵敏度设计

Spark的发光模块可为 96孔、384孔和1,536孔的微光、闪烁和多色发光应用提供更高的灵敏度。前沿的发光光路设计,可以在保持灵敏度的同时记录光谱,让您获得出色的检测性能,同时又可为未来的发光应用未雨绸缪。

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Glow luminescence linearity of the of the Spark using optimized conditions.

Optimized optics = optimal results

Spark uses luminescence fibers optimized for each plate format – 96, 384 and 1,536 wells – guaranteeing minimum signal crosstalk between wells. The light is guided through an innovative filter wheel consisting of multiple attenuation filters – reducing high signals by a factor of up to 1,000 – and 38 user-selectable colored filters, enabling virtually any multi-color luminescence measurement. The resulting signal is detected by a photomultiplier tube (PMT) operated in its most sensitive mode; photon counting.

This unique combination of optical components ensures maximum sensitivity for weak luminescent signals and allows attenuation of high signals. The result is an industry-leading dynamic range of 109, combined with exceptionally low detection limits for glow and flash luminescence.

Emission spectra of Renilla (RR) and firefly (FF) luciferases recorded with the Spark

Optimize your assay with multi-color luminescence

Most plate readers use a fluorescence emission monochromator for luminescence multiplexing or scanning, leading to inferior results and an unacceptable drop in sensitivity due to attenuation.

To overcome this limitation, Spark uses a unique filter technology based on 19 pairs of short- and long-pass filters. With just a few clicks, you can select the desired wavelength and bandwidth for multi-color luminescence measurements or spectral scans. This makes Spark a future-proof set-up for multiplexed assay systems (such as Chroma-Glo™), BRET applications – including NanoBRET™ – and luminescence spectral recording.

The Alpha reader of choice

Flawless performance of Alpha Technology assays requires high quality instruments. Spark combines a high power laser light source with an innovative filter wheel and dedicated photon counting PMT for ultra-sensitive detection of Alpha assays. 

Spark is equipped with a unique IR temperature sensor enabling well-by-well temperature correction during measurements, overcoming a well-known challenge of Alpha assays. This is complemented by Spark’s Te-Cool™ module for stable temperature management, even below ambient, giving you full control over this critical assay parameter.

These features ensure Spark offers uncompromised performance for AlphaScreen®, AlphaLISA® and AlphaPlex® assays, as well as potential future Alpha Technology applications.

自动化细胞成像

实现细胞计数、活性分析和活细胞成像

在多功能酶标仪之中直接内置简单、高性价比的细胞成像功能。Spark细胞成像模块包括一个LED 明场发光装置、一个4x物镜、一个CMOS 130万像素摄像机和一个牢固的激光自动对焦系统,可提供堪比自动化显微镜的性能。对于希望进入这一激动人心的应用领域的用户,该系统可提供经济的解决方案。

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Cell counting and viability

Cell counting and cell viability assessment of trypsinized cells is a daily task in any cell culture lab. Spark’s cell imaging module offers automated label-free cell counting and trypan blue-based cell viability analysis with an easy-to-use, disposable Cell Chip. Each chip has two sample chambers which can accommodate 10 μl of cell suspension, and four Cell Chips can be loaded onto the Cell Chip adapter, allowing eight samples to be analyzed in less than two minutes.

This user-friendly solution offers: 

  • High throughput, label-free cell counting, even at low cell concentrations
  • One-click live/dead analysis
  • High precision replicate processing for multiple samples
  • Fast and simple mean value calculations, greater accuracy and more precise data

Histogram view to enable adjustment of the analysis based on cell size for the study of sub-populations, eg. for cell swelling assessments

Live cell imaging and confluence

In general, you don’t know how many cells have adhered to the bottom of a microplate well, or even grown, over a certain period of time. Determination of cell confluence and estimation of cell numbers are therefore important for reproducible assay results and interpretation of experimental data.

With Spark’s cell imaging module, you can automatically assess the precise confluence – from 10-90 % –  giving you a new level of data quality in your assays.

Key applications:

  • Visual quality control of cells in microplates – an automated microscope in your reader
  • Label-free cell proliferation – detect cell growth in real time, without manual interference
  • Better assay consistency – always start your assay at the same confluence level, increasing the reproducibility of your cell-based assays
  • Signal normalization – use confluence data to normalize your cell-based signals to the cell mass in your well
  • Quality control – prove the optical quality of your microplate using confluence assessments
  • Wound healing studies– perform semi-automated cell migration and wound healing assays with comparable performance to a dedicated imaging device
  • Single cell cloning – combine automated image acquisition with semi-automated image analysis to verify monoclonality

活细胞工具包

简单的完整环境控制

作为细胞生物学家,您每天的生活就是忙于时刻保持细胞“快乐”。 通过自动化的工作流程,您可以腾出自己被夺走的时间,又能让细胞“快乐”生长。Spark将自动化培养箱、酶标仪及细胞成像功能整合在一起,为您自己及您所在的实验室提供了更多新的可能。

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Automated incubator

Spark is equipped with precise temperature control to enable uniform heating of you microplate. To help ensure even temperature distribution, the microplate chamber is thermally isolated from the optics, guaranteeing stable and precise temperature regulation for your assays.

Spark adds another layer of environmental controls by putting CO2/O2 partial pressure control at your fingertips. An integrated Gas Control Module (GCM™) offers CO2 regulation from 0.04 to 10 % (vol.), and O2 from 0.1 to 21 % (vol.), creating an ideal environment for your cells. CO2 regulation maintains cell health during long-term kinetic studies, while O2 control allows you to maintain the correct atmosphere for microaerophilic organisms or mimic physiologic conditions.

Generating growth curves with bacteria or yeast also requires effective plate shaking. Based on years of experience and knowledge of working with cell lines in microplate readers, Spark was specifically engineered for long-term shaking and incubation of micro-organisms.

Easy-to-use SparkControl software gives you straightforward control of these features, placing the best conditions for your live cell assays in your hands.

Humidity control – protection from edge effects

Humidity regulation at levels of 95 % or higher is another key element for good cell viability and growth, and minimizing evaporation is essential for maintaining constant concentrations during long-term assays. Spark’s Humidity Cassette is a cost-effective solution to minimize evaporation in any SBS-format microplate. This patented tool incorporates a filling moat and has a specialized lid for use with our unique Lid Lifter, giving you passive humidity control for any assay. Simply add ddH2O to the filling moat and you have an instant, no-fuss humidified atmosphere in your microplate chamber.

Lid Lifter

Spark gives you access to a new type of automation – integrated lid lifting – creating new workflow possibilities, reducing the risk of sample contamination and helping to establish an ideal environment for long-term kinetic assays. Whether you want to dispense compounds without the need for manual intervention or maintain optimal environmental conditions without comprising evaporation protection, Spark is the only reader up to the task.

Software designed for scientists

SparkControl combines automation of long-term kinetic assays with advanced features such as kinetic conditioning, remote operation and Open Kinetic, providing a hands-off solution for complex experimental set-ups.

Many live cell kinetic experiments require specific actions – for example, addition of a compound – once a certain signal level or OD value is reached. Multimode readers equipped with manual environmental controls can make these types of study cumbersome or error-prone. SparkControl’s kinetic conditioning feature allows you to program the system to automatically perform a required action as soon as a predefined signal is reached in a control well, fitting almost any assay workflow you can think of, in un-lidded or lidded microplates.

SparkControl’s remote control feature allows you to monitor or operate the instrument from outside of the lab. This enables full system control without the need to sit in front of the reader for hours; if you are connected to the internet, then you are connected to your experiment.

A multimode reader is frequently used for multiple applications by various users. Long-term live cell kinetics assays can block the reader for an entire day, limiting overall lab throughput. SparkControl offers an efficient solution: Open Kinetic. This feature allows you to pause and resume kinetic experiments, enabling additional measurements to be performed throughout long-term studies. This frees up the instrument for other users, eliminating one of the major bottlenecks of shared lab equipment, without compromising your valuable research.

Te-Cool – 真正的温度稳定性

整体温度控制 - 即便在低于环境温度的情况下

一致的温度是确保获得可靠结果的先决条件。Spark是首款内置冷却功能的多功能酶标仪,将整体温度控制在18到42 °C之间。

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Biology is not always at 37 °C

The target temperature isn’t always 37 °C in all biological situations. For example, zebra fish larvae need to be at 25 °C, and insect cells (Sf9) require 22 °C. A typical multimode reader can only heat the microplate chamber, usually requiring an offset of 2-5 °C above the current room temperature. This traditional approach to temperature control eliminates flexibility and will result in sub-optimal experimental conditions, leading to unreliable or unusable data. This lack of functionality also limits your ability to investigate certain organisms or applications.

With Spark’s unique Te-Cool module, you can heat or cool the measurement chamber. This enables you to set any user-defined temperature from 18 to 42 °C, guaranteeing stable temperature conditions

Exemplary luminescent kinase assay, where the activity of a glow luciferase was measured at different temperatures. The luminescent signal increased approximately 10 % per 1 °C increase in temperature.

Impact of temperature on luminescence

Temperature plays a crucial role on biochemical assays using enzymes derived from non-mammalian organisms. For example, bioluminescenct assay systems require optimal temperatures between 20 and 25 °C. These assays are usually highly temperature sensitive, with even a 1 °C increase in temperature leading to a 10 % increase in luminescence output. Luminometers or multimode readers that cannot stably control temperature in this critical range will therefore generate irreproducible data.

Spark is the only reader that can mitigate the impact of temperature on your luminescence assays, yielding higher quality data for your valuable research.

Cool down in a snap

Multiple users require multiple experimental conditions. A major bottleneck for microplate readers is waiting for an instrument to reach the desired temperature. Heating up the measurement chamber is easy, but what if you have to wait for it to cool down?

It typically takes over 60 minutes for a reader to cool down from 37 °C to room temperature through passive cooling, costing you valuable time and disrupting your busy schedule. Imagine the time delays and decrease in productivity you could avoid in a multi-user lab by just shortening that cool-down window.

The Te-Cool module gives you active cooling of the microplate chamber, resulting in significant time savings. Labs can effectively reduce their cool-down time going from 37 to 22 °C.

Luminescent signal over time in a kinetic measurement. During a measurement time of 6 min, the luminescent signal increases due to heating of the liquid in the wells).

Prioritize your valuable resources

Researchers in every lab are constantly being pushed and pulled to make the next discovery on limited assets. Whether the constraint is driven by budget or time, we have all felt the burden.

Spark brings together a unique set of features intended to ease this strain and create new workflow possibilities for your laboratory, reducing hands-on time while producing high quality data for each and every sample.

Whether you are looking to design long-term experiments that work around your schedule, attempting to shrink sample volumes to reduce costs, or trying gain access to multiple instruments on a tight budget, Spark gives you control over your priorities. 

Eliminate data variability at the source

As scientists, we consider ourselves data-driven. Whether your research is centered in academia or industry, your next discovery will rely on your data. The ability to generate reliable, reproducible and high quality data should be a prerequisite for all laboratory instruments.

Every time you run an experiment you invariably introduce some level of error that impacts your results. Variability is hard to avoid, whether it is transferring your cell-based assay between instruments or manually assessing confluence. Whether the errors are human subjectivity, environmental factors or contamination, you are influencing your results. Completely eliminating this variability isn’t realistic, so you are forced to make concessions. But isn’t your data too important to compromise?

Spark is designed to address almost every detail of the most demanding assays and applications, delivering technologies that seamlessly work together to allow you to generate accurate and consistent data, each and every day.

SparkControl™ 适应您的检测需要

SparkControl擅长处理常规或复杂工作流程。无论您需要定量某种核酸,还是要以特定的吸光值自动化加样, SparkControl 均能胜任,帮助您腾出更多的时间进行自己的研究。

可用性处于中心地位

真正的简便性

一键式应用程序可简化您的大多数检测,让您以前所未有的速度从样品获得结果。SparkControl采用图标驱动、“拖放式”操作,适于任何技术水平的用户。

轻松实现复杂应用

SparkControl基于工作流程的界面,允许您即刻访问应用程序并享受轻松的操作。该软件允许用户轻松将现有实验方案转化为可随时运行或编辑的操作方法。

视频

Open Kinetic

不用再担心注册表单。

Open Kinetic allows you to pause and resume kinetic reads. In the case of long-term experiments or experiments with long reading intervals, this feature enables other users to perform separate applications between kinetic measurements.

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实时浏览器

最近一次拍摄的影像。SparkControl让Spark变成了一个显微镜。

Live Viewer allows the user to monitor cells under optimal or physiological conditions in real-time.

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内置荧光团文库

包含精确光谱的荧光团列表,让您快速可视化,轻松选择激发和发射波长。

Integrated flurophore library with more than 60 fluorophores allows easy and precise visual selection of emission and excitation wavelengths.

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Highlights

  • 一键式应用程序
  • 环境控制
  • 最佳的读取效果
  • 自动化Z-聚集
  • 高清孔扫描
  • 高分辨率 3D 扫描
  • 扩展的动态范围
  • 动力学检测保护
  • Magellan

高分辨率 3D 扫描

加速您的检测开发

High resolution 3D scanning provides simultaneous excitation and emission scans, giving you the ability to quickly identify changes in the spectral properties of fluorescent probes and characterize unknown fluorescent samples.

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扩展的动态范围

检测极低信号

Automatic adjustment of gain settings during a measurement means you never have to compromise on sensitivity, allowing the detection of very low signals.

SparkControl takes care of everything, correlating and displaying all of your results in one single data set.

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自动化Z-聚集

更高的荧光灵敏度

Significantly improve the quality of your results with enhanced sensitivity in top reading fluorescence intensity and fluorescence polarization assays.

The automated z-focus adjustment determines the focal point for the best signal-to-blank ratio in each microplate well, regardless of the plate type, well shape or sample volume.

This unique feature makes it easy to set up your reader for optimum performance with varying assay parameters.

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一键式应用程序

以前所未有的速度从样品获得结果

SparkControl’s one-click applications epitomize our effort to put fast, simple instrument operation at your fingertips.

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高清孔扫描

完整的细胞群图像

Obtain a more accurate signal from each well, even with inhomogeneous cell layers.

High definition well scans give you a qualitative image of the cell distribution, effectively giving you a complete picture of the cell population.

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环境控制

一键调节条件

In combination with Spark’s unique environmental control options – including the GCM™ and Te-Cool™ module – SparkControl makes it easy to adjust parameters on the fly.

You have full control of the environmental conditions during a run, including the temperature (even below ambient) and the CO2 and O2 levels inside the reader.

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最佳的读数荧光测量

优化的底部荧光读取

SparkControl features a special Optimal Read (OR) function designed to improve cell-based measurements. OR delivers increased sensitivity for enhanced FI bottom reading by performing multiple measurements on spatially separated spots arrayed across each well.

This allows you to achieve very low CVs with high intra- and inter-well reproducibility when measuring adherent cells in microplates.

Optimal Read fluorescence measurements - Diagram

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动力学检测保护

为您的检测提供安全防护

Safeguard your kinetic assays using automated gain regulation to avoid fluorescence measurements running into saturation.

Measurements with different gain settings are then automatically correlated, allowing evaluation of the entire dataset.

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SparkControl Magellan™

增强您的数据分析能力

SparkControl Magellan is a data analysis package that provides you with powerful data reduction tools for numerous detection modes.

You gain access to an array of features designed to enhance functionality, simplicity and security for a variety of microplate-based applications.  

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Spark选配件

带加热和搅拌的自动液体加样器

综合加样器使您具备了极佳的加样能力,非常适合高灵敏度应用,如闪光。独特的加热和搅拌功能,减少了可预知的风险。

Spark-StackTM微孔板堆栈

Spark-Stack是一款结构紧凑的自动化微孔板堆栈,目的在于整合到Spark酶标仪当中。它可以为实验提供极其可靠的无人值守处理,每次运行可容纳多达50个微孔板。

QC 工具 (IQ/OQ 服务)

整个寿命周期内的可靠通量。Tecan的质量控制组合包可帮助您的Tecan酶标仪高效、高性价比地符合所有监管要求。

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挥发保护(湿度盒)

内置蒸发保护功能可以增强活细胞动力学检测,最大限度降低边缘效应,获得更具可重复性、更可靠的数据。

A patented Humidity Cassette reduces evaporation in standard microplates, enabling long-term live kinetic studies without the need to switch to dedicated and costly microplate types – simply use your current, validated plates. Benefits include:

  • Reduction in evaporation during your assay
  • Use your existing plates – no need for expensive special plates
  • Better data quality – consistant results
  • Reduced edge effects – ideal for long-term kinetics
  • Stable conditions – better cell viability
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为细胞计数而生的Cell Chips™

Tecan创新的Cell Chips(细胞芯片)最大限度减少了样品制剂的准备工作,带来更大的应用灵活性,为悬浮细胞的计数开启了新机会。

With its built-in cell counter, Spark can analyze a broad range of cell sizes and types, providing fully automated, label-free cell counting and analysis in less than 30 seconds.

  • Accurate, reproducible cell counting, with flexible area selection for greater sensitivity
  • One-click analysis of cell number, size distribution and viability
  • Export images for visual confirmation
  • High precision cell counting, even at low concentrations
  • Automated replicate processing for multiple samples in disposable Cell Chips
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NanoQuant Plate™

NanoQuant Plate允许同时定量和分析低至 2 µl 至多16种核酸或蛋白质样品。NanoQuant Plate的独特设计,每份样品选择1/4,消除了校准需要,并可保证一致的性能。

Brochure

Spark 多功能微孔板检测平台,可为几乎所有药物开发和前沿生命科学研究提供量身定制的解决方案。研究人员可根据当前需求在此平台上自由配置功能模块,并可通过升级以获取未来所需的其它技术和特点。

Spark-Stack™ plate stacker for walkaway automation of Tecan Spark® readers Enhance the productivity of your lab with this compact, reliable and field-upgradeable plate stacker. Spark-Stack takes care of typical process steps, such as loading, unloading and re-stacking of non-lidded 6- to 1,536-well plates, and provides a throughput of up to 50 plates per run.

Application Notes

The Optimal Read function for fluorescence bottom measurements is designed to enable uniform well illumination during excitation while and whole-well emission for 6-to 384-well plates. Performing multiple measurements on spatially separated spots arrayed across each well ensures that even heterogeneous cell layers can be effectively and reproducibly analyzed. This application note shows that the Optimal Read function produces the most reliable results for the analysis of adherent cell types, giving the highest well-to-well uniformity and best sensitivity for cell-based applications.

Cell counting, viability analysis and determination of size distribution are routinely performed by researchers across multiple disciplines, from standard cell passaging for downstream experiments to using insect cells infected with baculovirus to produce recombinant proteins, or determining the differentiation capacity of stem cells. Tecan’s Spark multimode reader has an integrated, bright field cell imaging module. This patent-pending system enables label-free cell counting, size distribution determination and trypan blue-based cell viability analysis with an easy-to-use, disposable Cell Chip™. In this study, the analysis of cell concentration, cell viability and size distribution for the determination of the optimal protein production rate in recombinant Sf21 cell cultures is presented.

HTRF is a time-resolved fluorescence resonance energy transfer (TR-FRET)-based assay system from Cisbio Bioassays, and is becoming increasingly popular for the analysis of various molecular interactions and binding studies. HTRF assays place high sensitivity demands on multimode readers. The Spark offers a number of features to meet these demands and to ensure optimal assay performance, including Tecan’s unique Fusion Optics which combine the sensitivity of filters and the flexibility of monochromators (MCRs) in one instrument. In addition to entirely filter- or MCR-based measurements, a combination of the two can be used simultaneously within one measurement.

This application note describes the successful implementation of AlphaScreen on the Spark multimode reader, as well as the performance of the instrument with regards to measurement sensitivity, uniformity and typical read times.

This application note describes the implementation of the AlphaScreen technology on the Spark multimode reader and the detection of tyrosine kinase activity using the AlphaScreen P-Tyr-100 Assay Kit.

This application note describes the implementation of the AlphaLISA technology on the Spark multimode reader and its use for the detection of human immunoglobulin G (IgG) using the AlphaLISA IgG Assay Kit.

Tecan’s Spark multimode microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cel lconfluence, thereby boosting automation of cell-based studies.

Keep control of crucial experimental conditions with the first air conditioning system for a multimode reader – enabling stable measurement chamber temperature down to 18°C.

Assaying cells in an appropriate window of time after a compound-­mediated cytotoxic event can be extremely challenging. This note describes the automation for kinetic monitoring of cytotoxicity and viability using three Promega assays for viability, cytotoxicity and apoptosis assessment with the Spark multimode reader.

Cell migration is a central process in the development and maintenance of multicellular organisms, with tissue formation during embryonic development, wound healing and immune responses all requiring the orchestrated movement and assembly of cells.

Tecan’s Spark multimode microplate reader was used for the real-time analysis of human stem cell proliferation and differentiation, using established assays for cell viability, proliferation and apoptosis assessment in a reliable and convenient manner.

Tecan’s Spark multimode microplate reader was used to identify metabolic changes during the differentiation of human mesenchymal stem cells and to assess and quantify cell viability, cytotoxicity and apoptosis, using several well-established and reliable cell-based assays developed by PromoCell.

For many cell biology applications – such as the identification and separation of high expression clones or knock-down candidates, and small-scale antibody or protein production – it is important to ensure that cell lines originates from a single cell, i.e. they are monoclonal. This can be a major bottleneck, especially for academic laboratories, as dedicated automated microscopy systems are both very expensive and complex to operate, meaning verification of monoclonality is often performed by manual microscopy.

Homogeneous Time-Resolved Fluorescence (HTRF) is a time-resolved fluorescence resonance energy transfer (TR-FRET) assay system developed by Cisbio Bioassays, and offers a reliable technique for high throughput screening (HTS) and the analysis of molecular interactions. The assay is based on energy transfer between a long-lived europium or terbium cryptate donor and a variety of red or green acceptors, and combines the advantages of FRET and time-resolved fluorescence (TRF).

Technical Notes

Spark-Stack is a compact, versatile and field-upgradeable microplate stacker for the Spark® multimode reader. It offers walkaway automation and enhances the productivity in the lab by helping to automate typical workflow steps – including plate loading, unloading and restacking of 6-1536-well microplates. Spark-Stack can be equipped with dark covers to protect light-sensitive assays inside the plate magazines, such as Alpha technology-based assays and fluorophore-transfected cells.

The one-click Live Viewer software application turns the Spark microplate reader into a microscope. It enables easy and straightforward visual quality checks of cells in microplate wells or in Tecan's proprietary Cell Chip™, allowing researchers to automatically acquire and instantly view images of their cell samples immediately before the measurement is started.

The Spark reader is equipped with Tecan's unique Fusion Optics, which combine the benefits of filters and MCRs in a single multimode reader. The Spark can be equipped with filters, Quad4 Monochromators or a combination of both using the ingenious Spark Fusion Optics.

The new Spark multimode microplate reader is equipped with patent-pending High Speed Monochromators (HSM) enabling highly accurate, reproducible and ultra-fast absorbance measurements. This unique optical system offers an enhanced measurement range from 200 to 1,000 nm, ensuring optimal performance across the full range, particularly for absorbance measurements in the deep UV range, such as A260/A230 nucleic acid purity checks. The Spark reader is also suitable for measurements of low volume samples in both absorbance and fluorescence modes using the patented NanoQuant plate.

With the Spark‘s luminescence module, users can perform high sensitivity flash and glow luminescence measurements. For optimized signal detection of very high and low signals within one experiment, optical density (OD) filters help to attenuate strong signals in the range from OD1 to OD3, thereby avoiding ‘over’ readings. This enables users to detect luminescence signals with a dynamic range of more than 9 orders of magnitude.Multicolor luminescence assays with up to five different luciferases, as well as luminescence spectral scanning (eg. of luminescent proteins) can be easily set - using an innovative filter-based approach, users can select any wavelength between 398 nm and 653 nm, with a step size of 15 nm and a 25 nm bandwidth.

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the maximum instrument sensitivity of the Spark multimode reader for flash and glow luminescence.

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the best possible detection limit for standard fluorophore label in fluorescence intensity top reading mode (FI top).

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the best possible detection limit of europium in time-resolved fluorescence intensity reading.

This technical note describes the optimized test set-ups, instrument settings and statistical equations that were used to determine the best possible detection limit for standard fluorophore label in FI bottom mode.

In this technical note, we demonstrate the superior performance of the Spark reader compared to two other multimode readers from different providers. The detection limit for the far red fluorescent dye Alexa Fluor 647 – which has a fluorescence spectrum comparable to Cy5 – was determined, operating each instrument in MCR mode.

This technical note describes the implementation of the DLR Assay System on Tecan’s next generation high-end multimode reader platform Spark. It can perform high sensitivity flash and glow luminescence measurements with a dynamic range of more than nine orders of magnitude. In addition, its enhanced luminescence module can measure multicolor assays with up to five different luciferases, as well as offering spectral scanning of luminescent substances.

This Technical Note describes the functions and benefits of the Spark multimode microplate reader and cell imaging module, as well as the SparkControl software and its dedicated Cell Counting and Cell Viability Application.

This technical note compares the performance of the Spark’s cell counting module with (i) manual cell counting using C-Chip™ disposable hemocytometers and a standard microscope, (ii) a comparably priced, imaging-based cell counting device (Device A) and (iii) a high performance cell imager with counting capabilities (Device B).

The Transcreener® ADP2 TR-FRET Red Assay is a competitive immunoassay for ADP with a far-red, time-resolved fluorescence-resonance-energy-transfer (TR-FRET) read-out. It is intended for the detection adenosine diphosphate (ADP) production by any kinase or ATPase, and has been developed specifically for high-throughput screening applications. The Spark was tested for its capacity to measure the Transcreener ADP2 TR-FRET Red Assay using the four different combinations of the Fusion Optics.

The Transcreener ADP2 FP assay is a competitive FP assay based on the detection of ADP and is therefore compatible with any enzyme class that produces ADP, including protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases and glutamine synthetase. This technical note describes the instrument settings for the Transcreener ADP2 FP assay on Tecan’s Spark multimode microplate reader.

Posters

Tecan’s Spark multimode microplate reader with integrated cell imaging simplifies cell culture, featuring quality checks and signal normalization to cell confluence, thereby boosting automation of cell-based studies.

Keep control of crucial experimental conditions with the first air conditioning system for a multimode reader – enabling stable measurement chamber temperature down to 18°C.

Cell-based assays are very popular in life sciences research, as they represent an in vitro system which offers results comparable to in vivo systems. However, this improved biological relevance is counterbalanced by the complexity of working with a living organism. Cell-based assays therefore require special precautions and care when planning and conducting experiments

Tomorrow´s imaging multimode plate reader with Smart Automation

Tecan has launched the Spark® multimode microplate reader, offering tailor-made solutions to suit virtually any drug discovery or advanced life science research application. This freely configurable system gives researchers access to new techniques and features intended to enhance and streamline biochemical and cell-based workflows.

At the heart of the instrument are Spark’s unique Fusion Optics and a powerful, ultra-high frequency xenon flash lamp, which can be combined with your choice of high performance detection modules to ensure a perfect match for your workflow.

Options include an enhanced fluorescence module designed to improve sensitivity and increase the speed of high throughput screening, and the newly developed Te-Cool™ cooling module that uniquely allows the measurement chamber temperature to be set below the ambient room temperature for more accurate and reliable results.

The Spark cell handling capabilities have also been extended, with a new automated cell imaging and confluence measurement feature. This allows incubation and monitoring of cell culture microplates within the measurement chamber, enabling the user to define the confluence at which the assay starts/ends or the substrate is injected, for optimal assay results and greater walkaway operation. These new options – combined with the ability to read 1- to 1,536-well microplates – ensure there is a Spark configuration to increase the productivity of your lab, both today and in the future.

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网络研讨会视频

无人值守的情况下观察细胞毒性、活细胞和死细胞

了解自己的细胞分析工作流程中的细胞活性和细胞毒性检测如何实现简化和自动化 。本网络研讨会焦点为,使用Promega 细胞健康检测和Tecan Spark 多模酶标仪评估细胞毒性、活性细胞和死细胞情况,包括多天的细胞动力学(或实时)分析。

通过评估细胞迁移和细胞活性,提高伤口愈合检测可信度

应用科学家Katrin Flatscher博士和Tecan检测市场经理Michael Fejtl博士将介绍Spark®多模酶标仪成像功能在检测细胞迁移方面的应用。

自动化检测人类细胞分化、增殖及代谢活动

讨论组成员Rüdiger Arnold博士、Katrin Flatscher博士和主持人 Michael Fejtl的这次长谈,将重点关注如何采用PromoCell开发的细胞检测方法及Tecan Spark酶标仪进行的人干细胞分化实时分析,以实现对细胞活性、增殖或死细胞情况快速、方便、灵敏、可重复地评估和定量。

Battle the heat in your lab - facing distorted results caused by temperature fluctuations

Dr Gediminas Vidugiris of Promega and Dr Katrin Flatscher of Tecan talk about the temperature susceptibility of many biochemical assays and how temperature fluctuations in the lab can distort measurement results. They discuss ways to achieve stable temperature conditions throughout the entire experimental workflow using the Te-coolTM active cooling functionality in the Spark microplate reader. The benefits of active temperature control are demonstrated on the basis of experimental data from cell-based bioluminescent and biofluorescent assays assessing cell viability, metabolism, death and signaling.

应用视频

探索多模酶标仪内的管盖升降功能

Spark让您可以无需手动干预,使用试剂加热仪和带盖微孔板提升效率。在长时间动力学运行期间,可以通过内置的板盖升降装置,自动去除酶标仪内部的微孔板盖,进行成分加样。

通过“Spectra Viewer”优化您的检测读取

Spark Control内的一种软件工具可帮助您优化所用荧光团的激发和发射设置。

一键式细胞计数和活性检测

Spark细胞染色模块采用易用的一次性Cell Chip™芯片。实现自动化、无需标记的细胞计数和台盼蓝染色法细胞活性分析。

通过“动力学调节”实现对动力学运行完全控制

Spark Control 内的 “动力学调节”软件功能支持自动化设置操作,例如,在指定的对照孔收到某一信号时,启动另一检测模式。

如何在5秒内获得完整的吸收光谱

Spark高速单色仪的创新设计,让您可以在短短5秒内获得200-1000 nm的吸收光谱,且最小增量不过1 nm。

如何通过几次鼠标点击记录高质量的发光光谱

多色发光光路的创新设计,使您可以在保持仪器灵敏度的情况下对发光信号进行光谱记录。

如何将Spark变成一台显微镜

SparkControl把Spark变成了一台显微镜。通过一键式实时浏览应用程序, 即可用4x放大率查看和捕获Cell Chip 或微量滴定板上指定位置的图像。在开始检测前,即可再查看一下您的反应体系。

自动化伤口愈合和细胞迁移

通过Spark reader,对人胆囊癌细胞进行30多小时的Radius™ 96孔细胞迁移检测(5%CO2,37°C,保湿卡盒),弥补了这一空白。

自动化活细胞成像

在Spark酶标仪上,进行50小时内的表皮样癌细胞自动化活细胞成像或汇合度评估(5%CO2,37°C,保湿卡盒)

阅读Spark宣传册

宣传册

Spark多功能酶标仪提供了几乎适合所有生命科学研究和药物研发应用的解决方案

阅读Spark宣传册
参见技术参数

技术参数

Spark 仪器规格及技术参数

参见技术参数

仅供研究使用。